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T0565

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

TMB membrane substrate, chromogenic, liquid

Synonym(s):

TMB membrane substrate

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.83

Product Name

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes, ready to use solution

Quality Level

100

form

liquid

storage temp.

2-8°C

Related Categories

General description

3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate for horseradish peroxidase (HRP) conjugates. It develops a permanent, insoluble, dark blue reaction product and is useful in colorimetric quantification.

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used as a chromogenic substrate in western blot analysis. It has also been used as a liquid substrate in enzyme-linked immunosorbent assay (ELISA).
3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes has been used in:
  • enzyme-linked immune absorbent spot (ELISPOT) assay
  • as a substrate for IgG peroxidase
  • in the visualization of immunocomplexes

Physical form

Ready-to-use.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
0000391229
0000391229
0000358193
0000392439
0000376027

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Navilla Apú et al.
Open life sciences, 18(1), 20220577-20220577 (2023-08-17)
Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use enzyme-linked immunosorbent assays (ELISA) or chemiluminescence immunoassays (CLIA); however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests
Muhammed M Salahuddin et al.
Current issues in molecular biology, 43(3), 2199-2209 (2021-12-24)
Breast cancer is the most common malignancy in women worldwide. P2X7 is a transmembrane receptor expressed in breast cancer and activated by the ATP tumor microenvironment, driving cell proliferation, angiogenesis, and metastasis via different signaling pathways. The role of the
Testin (TES) as a candidate tumour suppressor and prognostic marker in human astrocytoma
Steponaitis G, et al.
Oncology Letters, 12(5), 3305-3311 (2016)
immune response of multiparous hyper-immunized sows against peptides from non-structural and structural proteins of PRRSV
Rascon-Castelo E, et al.
Vaccines, 3(4), 973-987 (2015)
Hesham Saeed et al.
Preparative biochemistry & biotechnology, 52(6), 668-680 (2021-10-07)
Microbial L-asparaginases are aminohydrolases that hydrolyze L-asparagine to L-aspartate. They are used to treat acute lymphoblastic leukemia and Hodgkin's lymphomas and in food industries. Increasing demand for L-ASNases is therefore needed. In the current study, the recombinant L-ASNase from Dickeya
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