Analytical Method: Analytical Quality Assurance, Standard for Hydrogen peroxide

Reagents:
Prod. No. 109122 Potassium permanganate solution 0.02 mol/l
Prod. No. 107209 Perhydrol 30 % for analysis
Prod. No. 100716 Sulfuric acid 25 % for analysis
Prod. No. 116754 Water for analysis

Preparation of a stock solution:
Place 10 mL of Perhydrol 30 % in a calibrated or conformity-checked 100-mL volumetric flask and make up to the mark with distilled water. Transfer 30 mL (full pipette) of this solution to a calibrated or conformity-checked
1000-mL volumetric flask and make up to the mark with distilled water. The stock solution prepared according to this procedure has a concentration of approximately 1000 mg/L hydrogen peroxide.

Precise assay of the standard solution (stock solution) for hydrogen peroxide:
Pipette 50.0 mL (full pipette) of the hydrogen peroxide stock solution of approx. 1000 mg/Linto a 500-mL conical flask, dilute with 200 mL of distilled water, and add 30 mL of sulfuric acid 25 %. Titrate with a 0.02 mol/L potassium permanganate solution until the color changes to pink.

Calculation of the exact content of the hydrogen peroxide concentration:
Consumption of potassium permanganate (mL) x 34.02 = content of hydrogen peroxide, in mg/L

Further investigational concentrations may be prepared from the stock solution exactly determined according to the procedure described above by diluting accordingly.

Stability:
When stored in a cool place (refrigerator), the stock solution and the further diluted investigational solutions remain stable for one day.

For use with test kits:		Measuring range in mg/L
110011	MQuantTM Peroxide Test	5 - 50 H2O2
110081	MQuantTM Peroxide Test	1 - 100 H2O2
110337	MQuantTM Peroxide Test	100 - 1000 H2O2
116974	Reflectoquant® Peroxide Test	0.2 - 20.0 H2O2
116731	Reflectoquant® Peroxide Test	100 - 1000 H2O2
114731	Spectroquant® Hydrogen Peroxide Cell Test	2.0 - 20.0 H2O2 0.25 - 5.00 H2O2
118789	Spectroquant® Hydrogen Peroxide Test	0.15 - 6.00 H2O2

Materials

Product No.	Product Name	Description	Pricing
A4503	Bovine Serum Albumin	cold ethanol fraction, pH 5.2, ≥96%	Expand Hide
G7516	γ-Globulins from bovine blood	≥97% (agarose gel electrophoresis)	Expand Hide
I5523	Insulin from porcine pancreas	powder, ≥27 USP units/mg	Expand Hide
MABVN1250	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.2 μm, non-sterile	Expand Hide
MABVN0B50	MultiScreen-BV Filter Plate, 1.2 µm, opaque, non-sterile	Receptor binding assays, resin/bead based assays	Expand Hide
MDRPN0410	Multiscreen® 96 well Plate, PTFE membrane	pore size 0.45 μm, non-sterile	Expand Hide
MDRLN0410	Multiscreen® 96 well Plate, PTFE membrane	pore size 0.45 μm, non-sterile	Expand Hide
MADVN6550	MultiScreen-DV Filter Plate, 0.65 µm, clear, non-sterile	Receptor binding assays on whole cells and cell fragments, protein kinase/phosphatase assays, bead/resin based assays, sample prep	Expand Hide
MAGVS2210	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 0.22 μm, sterile	Expand Hide
MAHAS4510	Multiscreen® 96 well Plate, MCE membrane	pore size 0.45 μm, sterile	Expand Hide
MSBVN12	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.2 μm, non-sterile	Expand Hide
MSBVS1210	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.2 μm, sterile	Expand Hide
MSBVN1B50	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.2 μm, non-sterile	Expand Hide
MSDVN65	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 0.65 μm, non-sterile	Expand Hide
MSDVN6B50	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 0.65 μm, non-sterile	Expand Hide
MSFBN6B	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.0 μm, non-sterile	Expand Hide
MSFCN6B	Multiscreen® 96 well Plate, hydrophilic PVDF membrane	pore size 1.2 μm, non-sterile	Expand Hide
MSFCNXB50	Multiscreen® 96 well Plate, glass fiber membrane	pore size 1.2 μm, non-sterile	Expand Hide
MSHAN45	Multiscreen® 96 well Plate, MCE membrane	pore size 0.45 μm, non-sterile	Expand Hide
MSHAS4510	Multiscreen® 96 well Plate, MCE membrane	pore size 0.45 μm, sterile	Expand Hide

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