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574597-M

Millipore

Anti-Superoxide Dismutase (Cu/Zn Enzyme) Sheep pAb

liquid, Calbiochem®

Synonym(s):

Anti-SOD

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About This Item

UNSPSC Code:
12352203

biological source

sheep

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

mouse, rat, human, monkey

manufacturer/tradename

Calbiochem®

availability

not available in Canada,

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG

storage temp.

−20°C

target post-translational modification

unmodified

General description

Recognizes Cu/Zn SOD in red blood cells. Does not cross-react with Mn SOD.
Sheep polyclonal antibody purified by DEAE ion-exchange chromatography. Recognizes the Cu/Zn superoxide dismutase protein.
Superoxide dismutase Cu/Zn enzyme (SOD (Cu/Zn)) is a homodimeric metalloenzyme of aerobic organisms, comprised of subunits of ~16 kDa. Found in a variety of cell types, including erythrocytes, liver, and brain, it serves to protect cells against the oxidative damage of free radicals by catalyzing the conversion of superoxide anions (O2-) to molecular oxygen (O2) and hydrogen peroxide (H2O2).
This Anti-Superoxide Dismutase Sheep pAb is validated for use in WB, Immunoelectrophoresis, Frozen sections, Paraffin sections, Free-floating sections for the detection of Superoxide Dismutase.

Immunogen

Human
highly purified, human erythrocyte SOD Cu/Zn.

Application

Immunoblotting (1:1000)

Immunoelectrophoresis (see comments)

Frozen sections (see comments)

Paraffin sections (see comments)

Free-floating sections (see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In glycine-buffered saline, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human red blood cell lysate

Other Notes

Pasinelli, P., et al. 1998. Proc. Natl. Acad. Sci. USA95, 15763.
This antibody is monospecific as determined by immunoelectrophoresis against human red blood cell lysate and confirmed by immunodiffusion against a human red blood cell lysate and a known anti-SOD (Cu/Zn) antibody. Does not exhibit cross-reactivity with SOD (Mn) by double diffusion or with human serum. This antibody is reported to be suitable for use with free-floating, frozen, or paraffin embedded sections when used in immunohistochemistry. Variables assocated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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