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Key Documents

AB5354

Sigma-Aldrich

Anti-Internexin α Antibody

serum, Chemicon®

Synonym(s):

Anti-NEF5, Anti-NF-66, Anti-NF66, Anti-TXBP-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... INA(9118)

Specificity

alpha-internexin, C-terminal globular "tail" region. By western blot, AB5354 detects a major band at 66 kDa and a minor band at approximately 150 kDa corresponding to alpha internexin monomer and dimer, respectively. A minor band at approximately 50 kDa is occasionally detected and is believed to be a degradation product of alpha internexin. This antibody has been referred to in the literature as the R36 antibody (Evans, 2002).

Immunogen

Full length recombinant rat alpha-internexin fused to E. coli Trp E.

Application

Anti-Internexin Antibody, α is an antibody against Internexin for use in ELISA, WB, IC, IH.
Immunohistochemistry on formalin or paraformaldehyde fixed tissues.

Immunocytochemistry.

Western blot: using human or rat brain homogenates.

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Linkage

Replaces: 04-1032

Physical form

Serum liquid, containing no preservatives.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
POSITIVE CONTROL CELLS: PC12, Ntera2.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Motor unit abnormalities in Dystonia musculorum mice.
De Repentigny, Y; Ferrier, A; Ryan, SD; Sato, T; Kothary, R
Testing null
Kristi Lorenzen et al.
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Emerging evidence suggests that microglia can support neurogenesis. Little is known about the mechanisms by which microglia regulate the cortical environment and stimulate cortical neurogenesis. We used an in vitro co-culture model system to investigate the hypothesis that microglia respond
Pooja Shree Mishra et al.
Acta neuropathologica communications, 8(1), 65-65 (2020-05-10)
To test the hypothesis that the cerebrospinal fluid (CSF) could provide a spreading route for pathogenesis of amyotrophic lateral sclerosis (ALS), we have examined the effects of intraventricular infusion during 2 weeks of pooled CSF samples from sporadic ALS patients
Thomas A Ray et al.
eLife, 7 (2018-04-04)
A common strategy by which developing neurons locate their synaptic partners is through projections to circuit-specific neuropil sublayers. Once established, sublayers serve as a substrate for selective synapse formation, but how sublayers arise during neurodevelopment remains unknown. Here, we identify
Kuang-Wen Tseng et al.
Journal of neuropathology and experimental neurology, 65(4), 336-347 (2006-05-13)
Dystonia musculorum (dt) is a mutant mouse with hereditary sensory neuropathy. A defective bullous pemphigoid antigen 1 (BPAG1) gene is responsible for this mutation. In the present study, we examined the distribution of neuronal intermediate filament proteins in the central

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