ELI-008-M
IL-6 ELISPOT Antibody Pair, Mouse
from rat, clone MP5-20F3, clone MP5-32C11
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
clone
MP5-20F3, monoclonal
MP5-32C11, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable
shipped in
wet ice
General description
Interleukin 6 (IL-6) is a multifunctional cytokine that plays an important role in regulating immune and inflammatory responses. Produced by a wide variety of cells, including T cells, monocytes, fibroblasts, endothelial cells, and keratinocytes, IL-6 works by stimulating B-cell differentiation and antibody production, as well inducing expression of hepatic acute-phase proteins, regulating bone metabolism, and promoting neuronal differentiation. In addition, IL-6 appears to interact with IL-2 in the proliferation of T-lymphocytes and with IL-3 to promote the proliferation of multipotent hematopoietic progenitor cells. The Millipore mouse IL-6 ELISPOT Antibody Pair provides a sensitive and highly reliable method for the detection of IL-6 secreting cells at the single cell level.
Specificity
Both the capture and the detection antibodies included in this kit react with mouse interleukin -6 (IL-6).
Application
IL-6 ELISPOT Antibody Pair, Mouse is a highly specific rat monoclonal antibody & that targets IL-6 & has been tested in ELISA.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Packaging
Sufficient reagents for five, 96-well plates.
Components
• Mouse IL-6 Capture Antibody, clone MP5-20F3. Quantity: 500 µg.
• Mouse IL-6 Detection Antibody, clone MP5-32C11. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
• Mouse IL-6 Detection Antibody, clone MP5-32C11. Quantity: 100 µg.
• Streptavidin Alkaline Phosphatase. Quantity: 55 μL.
Target description
~25 kDa
Physical form
Format: Purified
The 500 µg purified IL-6 capture antibody is buffered in a preservative-free phosphate solution, pH 7.2, and the 100 µg purified IL-6 biotinylated detection antibody is buffered in a phosphate solution, pH 7.2, containing 0.09% sodium azide. Streptavidin alkaline phosphatase is supplied in a Tris Buffer, pH 8.0, containing 0.1 M sodium chloride, 1 mM magnesium chloride, 0.2% (w/v) BSA, 10% (w/w) glycerol, and 0.1% sodium azide.
Storage and Stability
Maintain refrigerated at 2-8°C for up to six months from the date of receipt.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Articles
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
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