HSTCMAG28SPMX13
MILLIPLEX® Human High Sensitivity T Cell Panel Premixed 13-plex - Immunology Multiplex Assay
Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Synonym(s):
Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel
About This Item
Quality Level
species reactivity
human
manufacturer/tradename
Milliplex®
assay range
accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)
standard curve range: 0.18-750 pg/mL
(IL-6)
standard curve range: 0.24-1,000 pg/mL
(IL-13)
standard curve range: 0.31-1,250 pg/mL
(IL-8)
standard curve range: 0.37-1,500 pg/mL
(IL-7)
standard curve range: 0.45-1,750 pg/mL
(TNFα)
standard curve range: 0.49-2,000 pg/mL
(IL-1β)
standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))
standard curve range: 0.49-2,000 pg/mL
(IL-2)
standard curve range: 0.49-2,000 pg/mL
(IL-5)
standard curve range: 0.61-2,500 pg/mL
(IFNγ)
standard curve range: 1.22-5,000 pg/mL
(GM-CSF)
standard curve range: 1.46-6,000 pg/mL
(IL-10)
standard curve range: 1.83-7,500 pg/mL
(IL-4)
inter-assay cv: <15%
intra-assay cv: <10%
technique(s)
multiplexing: suitable
compatibility
configured for Premixed
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
General description
The MILLIPLEX® Human High Sensitivity T Cell Magnetic Bead Panel is to be used for the simultaneous quantification of any or all of the following analytes in human plasma, serum, and cell/tissue culture supernatant samples: Fractalkine, GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-21, IL-23, ITAC, MIP-1α, MIP-1β, MIP-3α
and TNFα.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Cytokines/Chemokines
Specificity
There is no detectable cross-reactivity within the panel.
Application
- Analytes: GM-CSF, IFNγ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p70), IL-13, TNF-α
- Because there are unique features concerning sample prep and assay set-up, please read the entire protocol before running the assay.
- Recommended Sample type: Serum, plasma or tissue/cell lysate and culture supernatant
- Assay Run Time: Overnight
- Research Category: Inflammation & Immunology
- Research Sub-Category: Immune Response, Metabolic Disorders, Chronic Inflammatory Disease, Cardiovascular Disease, Neuroscience
Linkage
Storage and Stability
Other Notes
Legal Information
Disclaimer
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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