MAB1379
Anti-CD54 Antibody, extracellular, clone 84H10
clone 84H10, Chemicon®, from mouse
Synonym(s):
ICAM-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
84H10, monoclonal
species reactivity
human
should not react with
canine
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ICAM1(3383)
General description
This molecule is inducible to high levels by mitogenic lectins on lymphocytes and by IL-1beta or IFN gamma on other cell types such as fibroblasts and enthothelial cells.
Specificity
Recognizes the extracellular D1 domain of ICAM-1. Blocks binding of LFA-1 and P. falciparum to ICAM-1. Reacts with the ICAM-1 antigen found in low levels on lymphocytes and strongly expressed on monocytes and granulocytes. Detects an antigen of 90 kDa.
Immunogen
Epitope: extracellular
K562 cell line
Application
Detect CD54 using this Anti-CD54 Antibody, extracellular, clone 84H10 validated for use in FC, IP, IH.
Flow Cytometry: Neat - 1:10. 10 μL of suggested dilution will label 10e6 cells.
Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.
Immunoprecipitation: 5 μg/ 10e7 cells
Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.
Optimal working dilutions must be determined by end user.
Immunohistochemistry on frozen tissue sections: 1:20-1:50. Does not work on paraffin embedded tissue sections.
Immunoprecipitation: 5 μg/ 10e7 cells
Adhesion Blocking; blocks ICAM-1 mediated adhesion to LFA-1.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
Physical form
Affinity purified immunoglobulin. Liquid in Phosphate buffered saline, pH 7.4, containing 0.09% sodium azide.
Format: Purified
Storage and Stability
Store at 2-8°C for up to 12 months from date of receipt. Alternatively, this antibody can be stored at -20ºC. Avoid repeated freeze-thaw cycles.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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