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MAMIC5S10

Millipore

Multiscreen® 96 well Plate, polycarbonate membrane

pore size 5.0 μm, sterile

Synonym(s):

MultiScreen PC Plate, Polycarbonate 96-Well Plate

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About This Item

UNSPSC Code:
41104923
eCl@ss:
32039006
NACRES:
NB.22

material

flat bottom wells
polycarbonate membrane
polystyrene

Quality Level

400

description

Clear, Sterile, 5.0 µm pore size, Polycarbonate (PC) membrane, 10 plates

sterility

sterile

product line

MultiScreen®

feature

lid

manufacturer/tradename

MultiScreen®

parameter

50-250 μL sample volume (per well)

technique(s)

DNA purification: suitable

filtration area

0.28 cm2

plate size

96 wells

well maximum volume

350 μL

working volume

50-75 μL

pore size

5.0 μm pore size

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

General description

The MultiScreen®-MIC (Migration, Invasion, Chemotaxis) plate is a 96-well disposable device for use in functional assays for screening protein libraries and compound leads for drug discovery. It is designed to support high throughput cell-based functional assays such as migration, invasion, and chemotaxis with suspension and/or adherent cell lines, using a single device. The plates can be used to perform and quantify cell migration or invasion across coated or uncoated membranes in response to a concentration gradient. The MultiScreen-MIC plates are available in several pore sizes to accommodate MIC assays with a variety of cell lines and are intended for single use only.

Application

MultiScreen® Migration Invasion and Chemotaxis Filter Plate, 96 well has been used in the transwell/cellular migration assay using:
  • interleukin (IL)-4- and IL-12-treated T cells
  • THP-1 (human acute monocytic leukemia cell line)
  • in vitro activated, purified and IL-2-maintained galectin-3-deficient (gal3)-/- and gal3+/+ cluster of differentiation 4 (CD4)+ OTII T cells

Legal Information

MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Tilo Biedermann et al.
Journal of immunology (Baltimore, Md. : 1950), 177(6), 3763-3770 (2006-09-05)
Distinct pattern of homing receptors determines the tissue preference for T cells to exert their effector functions. This homing competence is mostly determined early during T cell activation of naive T cells. In contrast, mechanisms governing the acquisition of particular
Huan-Yuan Chen et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(34), 14496-14501 (2009-08-27)
We have investigated the function of endogenous galectin-3 in T cells. Galectin-3-deficient (gal3(-/-)) CD4(+) T cells secreted more IFN-gamma and IL-4 than gal3(+/+)CD4(+) T cells after T-cell receptor (TCR) engagement. Galectin-3 was recruited to the cytoplasmic side of the immunological
Jeremy Green et al.
Journal of medicinal chemistry, 58(12), 5028-5037 (2015-06-04)
The Rho kinases (ROCK1 and ROCK2) are highly homologous serine/threonine kinases that act on substrates associated with cellular motility, morphology, and contraction and are of therapeutic interest in diseases associated with cellular migration and contraction, such as hypertension, glaucoma, and

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