Skip to Content
Merck
All Photos(1)

Key Documents

NE1019

Sigma-Aldrich

Anti-Myelin Basic Protein Mouse mAb (SMI-99)

liquid, clone SMI-99, Calbiochem®

Synonym(s):

Myelin basic protein antibody

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

SMI-99, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

guinea pig (weakly), mammals

should not react with

chicken, porcine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MBP(4155)

General description

Purified mouse monoclonal antibody. Recognizes the ~21 kDa myelin basic protein (MBP).
Recognizes the ~21 kDa myelin basic protein in rat brain tissue. Does not recognize the 14 kDa form. Recognizes most mammalian species.
This Anti-Myelin Basic Protein Mouse mAb (SMI-99) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Frozen Sections, Paraffin Sections for the detection of Myelin Basic Protein.

Immunogen

Epitope: Within amino acids 131-136
human myelin basic protein

Application


ELISA (1:1000)
Immunoblotting (1:1000)
Immunocytochemistry (1:1000)
Frozen Sections (1:1000)
Paraffin Sections (1:1000)

Warning

Toxicity: Standard Handling (A)

Other Notes

Evers, P. and Uylings, H.B. 1997. J. Neurosci. Methods72, 197.
Shin, R.W., et al. 1991. Lab. Invest.64, 693.
Recognizes MBP from most mammalian species. Does not recognize the ~14 kDa form of MBP. Detects developing and adult myelin and developing oligodendrocytes. Also distinguishes oligodendrocytes from astrocytes, microglia, neurons, and other cells in brain tissue sections. In combination with Anti-Myelin Basic Protein Mouse mAb (SMI-91) (Cat. No. NE1020) and/or Anti-Myelin Basic Protein Mouse mAb (SMI-94) (Cat. No. NE1018) this antibody is useful for immunocytochemical studies on the progression of normal and pathologic myelination. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Jane L Zhu et al.
Journal of translational medicine, 20(1), 41-41 (2022-01-26)
Morphea is an autoimmune, sclerosing skin disorder. Despite the recent emphasis on immune dysregulation in morphea, the role of autoantibodies in morphea pathogenesis or utility as biomarkers are poorly defined. Autoantigen microarray was used to profile autoantibodies from the serum
Gerben van Hameren et al.
Acta neuropathologica communications, 7(1), 86-86 (2019-06-13)
Mitochondria are critical for the function and maintenance of myelinated axons notably through Adenosine triphosphate (ATP) production. A direct by-product of this ATP production is reactive oxygen species (ROS), which are highly deleterious for neurons. While ATP shortage and ROS
Yulia Grishchuk et al.
Disease models & mechanisms, 8(12), 1591-1601 (2015-09-24)
Mucolipidosis type IV (MLIV) is a lysosomal storage disease caused by mutations in the MCOLN1 gene, which encodes the lysosomal transient receptor potential ion channel mucolipin-1 (TRPML1). MLIV causes impaired motor and cognitive development, progressive loss of vision and gastric
Michela Losurdo et al.
Brain sciences, 10(4) (2020-04-16)
Traumatic brain injury (TBI) commonly results in primary diffuse axonal injury (DAI) and associated secondary injuries that evolve through a cascade of pathological mechanisms. We aim at assessing how myelin and oligodendrocytes react to head angular-acceleration-induced TBI in a previously
L N Collins et al.
The Journal of comparative neurology, 526(11), 1843-1858 (2018-04-18)
Both the lateral olfactory tract (LOT) and anterior limb of the anterior commissure (AC) carry olfactory information. The LOT forms the projection from the olfactory bulb to the ipsilateral olfactory cortices, while the AC carries odor information across the midline

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service