11088947001
Roche
BM Condimed H1
Hybridoma Cloning Supplement
Synonym(s):
hybridoma cloning supplement
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About This Item
Quality Level
sterility
0.2 μm filtered
form
solution
packaging
pkg of 100 mL (10x)
manufacturer/tradename
Roche
technique(s)
cell culture | hybridoma: suitable
solubility
water: miscible
storage temp.
−20°C
General description
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Cell Line Origin
BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.
Application
BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.
Features and Benefits
Contents
Solution in RPMI 1640, filtered through 0.2μm pore size membrane
Solution in RPMI 1640, filtered through 0.2μm pore size membrane
Physical form
Solution in RPMI 1640, filtered through 0.2 μm pore size membrane
Preparation Note
Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.
Analysis Note
Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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