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11922416001

Roche

Anti-His6

from mouse IgG1

Synonym(s):

metal chelate affinity chromatography

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

form

lyophilized

packaging

pkg of 100 μg

manufacturer/tradename

Roche

isotype

IgG1

storage temp.

2-8°C

General description

Anti-His6 is a monoclonal antibody to His6-tagged proteins. Anti-His6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured histidine-tagged fusion proteins independent of the location of the histidine-tag in the protein sequence.

Specificity

Anti-His6 specifically recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of location of the histidine-tag in the protein sequence. In addition, Anti-His6 preferentially recognizes the C-terminal His6-epitope with high sensitivity. Anti-His6 allows specific and sensitive detection of histidine-tagged proteins irrespective of the expression system used.
The antibody recognizes an epitope of six consecutive histidine residues (His6) in natural and recombinant proteins. It reacts with both native and denatured histidine-tagged fusion proteins, independent of the epitope-sequence location.

Application

Anti-His6 is used for the detection of His6-tagged proteins in:
  • Immunoblotting (e.g., dot blots and western blots)
  • Immunoprecipitation
  • Immunoassays (ELISA)
  • Immunocytochemistry
  • Immunofluorescence

Preparation Note

Stabilizers: BSA
Working concentration: Working concentration of conjugate depends on application and substrate.
The following concentrations should be taken as a guideline:
  • ELISA: for detection 0.1 μg/ml; for coating 1 to 5 μg/ml
  • Immunoprecipitation: 0.5 to 5 μg/ml
  • Western blot: 0.2 to 0.5 μg/ml

Reconstitution

Add 1 ml double-distilled water to a final concentration of 100 μg/ml.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H412

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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