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General description
Sepharose 2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
Application
Sepharose™ 2B has been used for the preparation of virosomes and platelets.
Legal Information
Sepharose is a trademark of Cytiva
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
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Depletion of glycoprotein gp85 from virosomes made with Epstein-Barr virus proteins abolishes their ability to fuse with virus receptor-bearing cells.
Journal of Virology, 63, 4998-4998 (1989)
Regulation of the phosphoinositide cycle by Na+/H+ exchange and intracellular pH in human platelets.
Biochimica et biophysica acta, 1084(1), 78-86 (1991-06-19)
We have found that thrombin-induced activation of protein kinase C (PKC) in platelets, measured by phosphorylation of the 47 kDa protein, is synergistically enhanced by the amiloride analogue ethylisopropylamiloride (EIA), a specific inhibitor of Na+/H+ exchange. This EIA effect was
Molecular Cloning and Physical Mapping of Potato Virus S Complementary DNA.
Phytopathology, 80, 446-450 (1990)
Langmuir : the ACS journal of surfaces and colloids, 28(5), 2745-2752 (2011-12-24)
Recent studies have shown that platelets can adhere to adsorbed albumin (Alb) through a receptor-mediated mechanism, but only if the Alb undergoes more than a critical degree of adsorption-induced unfolding. The objectives of this research were to investigate whether Alb
Cell, 17(2), 347-356 (1979-06-01)
A low molecular weight (LMW) protein phosphokinase enzyme that binds to actin has been isolated from murine sarcoma virions; this kinase activity is not present in nontransforming murine leukemia viruses. Sephadex G-75 gel filtration and affinity chromatography on actin-Sepharose conjugates
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