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W2261

Millipore

WL nutrient broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Wallerstein Laboratory Nutrient Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

bioburden testing
food and beverages
microbiology

suitability

selective for Candida spp.
selective for Escherichia coli
selective for Lactobacillus spp.
selective for Pichia spp.
selective for Proteus spp.
selective for Saccharomyces spp.
selective for Zygosaccharomyces spp.
selective for coliforms

Application

Recommended for the cultivation of bacteria encountered in breweries and industrial fermentations.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 5.00
Yeast extract, 4.00
Dextrose, 50.00
Monopotassium phosphate, 0.55
Potassium chloride, 0.425
Calcium chloride, 0.125
Manganese sulfate, 0.125
Ferric chloride, 0.0025
Manganese sulfate, 0.0025
Bromocresol green, 0.022

Preparation Note

Suspend 60.25 g of WL Nutrient Broth in 1000 ml of distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. If desired to obtain a pH of 6.5 add a 1% solution of sodium bicarbonate (Cat. No. 71627).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL3683
BCCK6010
BCCK9376
BCCH9492
BCBX4269

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Articles

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

Probiotics and Human Health

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Protocols

Yeast Growth Methods

Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

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