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01632

Sigma-Aldrich

Atto 520-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):

Biotin-Atto 520

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About This Item

Empirical Formula (Hill Notation):
C37H53ClN6O8S
Molecular Weight:
777.37
MDL number:
MFCD04040671
UNSPSC Code:
12352108
eCl@ss:
34058011
PubChem Substance ID:
329747336
NACRES:
NA.32

product line

BioReagent

Assay

≥90% (HPLC)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble
ethanol: soluble
methanol: soluble

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 522-528 nm Amax

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

[O-]Cl(=O)(=O)=O.CCNc1cc2OC3=CC(=[NH+]\CC)\C(C)=CC3=C(CCC(=O)NCCCCCNC(=O)CCCCC4SCC5NC(=O)NC45)c2cc1C

InChI

1S/C37H52N6O4S.ClHO4/c1-5-38-28-20-31-26(18-23(28)3)25(27-19-24(4)29(39-6-2)21-32(27)47-31)14-15-35(45)41-17-11-7-10-16-40-34(44)13-9-8-12-33-36-30(22-48-33)42-37(46)43-36;2-1(3,4)5/h18-21,30,33,36,38H,5-17,22H2,1-4H3,(H,40,44)(H,41,45)(H2,42,43,46);(H,2,3,4,5)/b39-29-;

InChI key

OCYRRKMPTKPPHX-QITHAKLPSA-N

Application

Atto 520 is a fluorescent label with high molecular absorption (110.000) and quantum yield (0.90) as well as sufficient stoke′s shift (excitation maximum 520 nm, emission maximum 542 nm). It does nearly not show cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Due to an insignificant triplet formation rate it is well suited for single molecule detection applications. Can be utilized in applications such as ELISA or immuno-histochemistry, in situ hybridization, flow cytometry and others, to identify streptavidin, avidin, or extravidin-conjugates. Atto-520 fluorescent-labeled biotin can be loaded onto magnetic beads coated with streptavidin through specific ligand–receptor interactions.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Atto Dyes for Superior Fluorescent Imaging

Atto dyes are a series of fluorescent dyes that meet the critical needs of modern fluorescent technologies.

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