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Key Documents

3010

Sigma-Aldrich

Transcreener® ADP2 FP Assay

Synonym(s):

Transcreener assay

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About This Item

UNSPSC Code:
41122100
NACRES:
NA.81

shipped in

dry ice

storage temp.

−20°C

General description

The Transcreener® HTS Assay platform overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by utilizing a single set of assay reagents that detect an invariant product. The generic nature of the Transcreener® HTS Assay platform eliminates delays involved in assay development for new HTS targets, and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® ADP2 FP Assay is a far-red, competitive fluorescence polarization (FP) assay based on the detection of ADP and therefore is compatible with any enzyme class that produces ADP, including protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases and glutamine synthetase. The Transcreener® ADP2 Assay is a simple one step homogenous detection assay, and is extremely flexible with regard to ATP concentration (0.1 to 1,000 μM ATP). The assay provides excellent signal at low substrate conversion, with a Z′ = 0.7 and = 85 polarization shift (mP) at 10% ATP conversion using 1 μM ATP.

The Transcreener® ADP2 FP Assay was developed to follow the progress of any enzyme that produces ADP. The Transcreener® ADP Detection Mixture comprises an ADP Alexa633 Tracer bound to an ADP2 Antibody. The tracer is displaced by ADP, the invariant product generated during the enzyme reaction.The displaced tracer freely rotates leading to a decrease in fluorescence polarization. The assay uses a far red tracer to minimize interference from fluorescent compounds and light scattering.

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Quantity

3010-1K = 1,000 assay, 384-well
3010-10K = 10,000 assay, 384-well

Physical form

Kit with buffered aqueous solutions

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nathalie Boone et al.
PloS one, 5(12), e15590-e15590 (2010-12-29)
Familial dysautonomia (FD) is a hereditary neuropathy caused by mutations in the IKBKAP gene, the most common of which results in variable tissue-specific mRNA splicing with skipping of exon 20. Defective splicing is especially severe in nervous tissue, leading to
Peng Su et al.
International journal of clinical and experimental pathology, 7(6), 3008-3017 (2014-07-18)
IMP3 plays an important role in tumor invasion and metastasis, to which epithelial to mesenchymal transition (EMT) also contributes. The purpose of this study was to investigate whether IMP3 can regulate invasion and metastasis through EMT in breast cancers. The
Founders of the first Hungarian Physiological Association in 1891.
E Monos et al.
Acta physiologica Hungarica, 97(1), 60-61 (2010-03-18)

Protocols

Measuring Kinase Inhibitor Residence Times

Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.

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