Skip to Content
Merck
All Photos(1)

Documents

B6024

Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

tablet, suitable for identification of lac+ bacterial colonies

Synonym(s):

5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C14H15BrClNO6
CAS Number:
7240-90-6
Molecular Weight:
408.63
Beilstein:
1402009
EC Number:
230-640-8
MDL number:
MFCD00005666
UNSPSC Code:
12352204
PubChem Substance ID:
57653950
NACRES:
NA.83

product name

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet

form

tablet

Quality Level

200

suitability

suitable for identification of lac+ bacterial colonies

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1

InChI key

OPIFSICVWOWJMJ-AEOCFKNESA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Biochem/physiol Actions

X-gal is routinely used to detect transgene activity in situ.

Quantity

Contains 5 mg substrate per tablet

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

related product

Product No.
Description
Pricing

94433

β-Galactosidase stain

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Creation of recombinant antigen-binding molecules derived from hybridomas secreting specific antibodies
Fields C, et al.
Nature Protocols, 8(6), 1125-1125 (2013)
Analysis of Transgene Expression
Transactions of the American Clinical and Climatological Association, 543-564 (2014)
Enzo R Porrello et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(1), 187-192 (2012-12-19)
We recently identified a brief time period during postnatal development when the mammalian heart retains significant regenerative potential after amputation of the ventricular apex. However, one major unresolved question is whether the neonatal mouse heart can also regenerate in response
Mohi Ahmed et al.
Development (Cambridge, England), 139(11), 1965-1977 (2012-04-20)
Inner ear neurogenesis depends upon the function of the proneural basic helix-loop-helix (bHLH) transcription factors NEUROG1 and NEUROD1. However, the transcriptional regulation of these factors is unknown. Here, using loss- and gain-of-function models, we show that EYA1 and SIX1 are
Daniel Grammel et al.
Acta neuropathologica, 123(4), 601-614 (2012-02-22)
Medulloblastoma is a malignant brain tumor of childhood that comprises at least four molecularly distinct subgroups. We have previously described that cerebellar granule neuron precursors may give rise to the subgroup with a molecular fingerprint of Sonic hedgehog (Shh) signaling.
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service