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C6137

Sigma-Aldrich

Chitinase from Streptomyces griseus

lyophilized powder (essentially salt free), ≥200 units/g solid

Synonym(s):

N-acetyl-glucosaminidasechitobiase, Chitin digestion enzymes, poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase

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About This Item

EC Number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder (essentially salt free)

Quality Level

specific activity

≥200 units/g solid

mol wt

30 kDa

solubility

H2O: soluble 0.90-1.10 mg/mL

storage temp.

−20°C

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General description

Chitinase is an extracellular complex of enzymes that degrade chitin. Chitin is a cell wall component of Fungi and exoskeketal essentials of different organisms which reshape their own chitin or digest/dissolve the chitin of other organisms (insects, fungi, yeast, and algae, and in the internal structures of other vertebrates) . Chitinases have been detected in many microorganisms and in plants. In fungi, chitinases assist in morphogenesis, to break down the inherent chitin content of fungal cell walls. Plant chitinases help in resistance to fungal attack and counteracting fungal growth, by targeting those same fungal cell walls. In bacteria, bacterial chitinases assist in utilizing chitin as a carbon source and as an energy source.Streptomyces griseus produces multiple chitinases of different molecular masses after growth induction with chitin as the carbon source.

The enzymatic hydrolysis of chitin to N-acetyl-D-glucosamine involves two consecutive enzyme reactions:
  • The first reaction, chitodextrinase-chitinase, is a poly(β-(1→4)-[2-acetamido-2-deoxy-D-glucoside])- glycanohydrolase, which removes chitobiose units from chitin.
  • The second activity is N-acetyl-glucosaminidasechitobiase, which cleaves the disaccharide to its monomer subunits, N-acetyl-D-glucosamine.

Application

Agriculture fields: control pathogens.
Human health care: Asthma.
Pharma: preparation of chitooligosaccharides and N-acetyl D glucosamine,
Preparation of single-cell protein
Isolation of protoplasts from fungi and yeast
Control of pathogenic fungi
Treatment of chitinous waste, mosquito control and morphogenesis

Biochem/physiol Actions

Chitinase is an extracellular enzyme complex that degrades chitin and has a molecular mass of approximately 30 kDa. Chitin is degraded to N-acetyl-D-glucosamine in 2 enzymatic reactions. Firstly, chitobiose units are removed from chitin by chitodextrinase-chitinase. The second reaction involves N-acetyl-glucosaminidase-chitobiase, which cleaves the disaccharide to its monomer subunits (that comprise of N-acetyl-D-glucosamine). The optimum reaction temperature is 37 °C.

Features and Benefits

Chitinase is an extracellular complex of enzymes that degrade chitin. It is a lytic enzyme suitable for fungal cell walls lysis.

Unit Definition

One unit will liberate 1.0 mg of N-acetyl-D-glucosamine from chitin per hour at pH 6.0 at 25 °C in a 2 hour assay.
One new 1 hour unit = approx. 50 old 48 hour units.

substrate

Product No.
Description
Pricing

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shigeo Suzuki et al.
International journal of biological macromolecules, 43(1), 13-19 (2007-11-06)
Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R
Vasiliki Skiada et al.
The New phytologist, 226(5), 1429-1444 (2020-01-31)
Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in
Melissa S Hillwig et al.
Journal of plant physiology, 168(7), 734-738 (2010-11-26)
Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To
Xinyu Liu et al.
Bio-protocol, 7(5), e2164-e2164 (2017-03-05)
Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as
Chitinase-resistant hydrophilic symbiotic factors secreted by Frankia activate both Ca(2+) spiking and NIN gene expression in the actinorhizal plant Casuarina glauca.
Chabaud, et al.
The New phytologist, 209, 86-93 (2020)

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