I4144

IL-3 mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-3, mIL-3, Interleukin-3 from mouse
• MDL number: MFCD00164817
• UNSPSC Code: 12352202
• NACRES: NA.77

Properties

• product name: Interleukin-3 from mouse, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• biological source: mouse
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• quality: endotoxin tested
• mol wt: 15.1 kDa
• packaging: pkg of 10 and 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1.000 EU/μg
• color: white to yellow
• UniProt accession no.: P01586
• storage temp.: −20°C
• Gene Information: mouse ... Il3(16187)

Description

General description

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

Application

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Physical form

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves