Skip to Content
Merck
All Photos(1)

Documents

L1900

Sigma-Aldrich

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

Synonym(s):

Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106200
NACRES:
NA.72

Quality Level

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26(1-2), 20-28 (2003)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26.0, 20-28 (2003)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service