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MAK126

Sigma-Aldrich

Bilirubin Assay Kit

sufficient for 180 colorimetric tests

Synonym(s):

Hematoidin Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 180 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder; gastrointestinal diseases

storage temp.

2-8°C

General description

Bilirubin, also known as hematoidin, is a degradation product formed as a result of heme catabolism in the liver. Bilirubin circulates in the blood stream as either the unconjugated insoluble form (indirect bilirubin) or the soluble glucuronide-conjugated form (direct bilirubin). Conjugated bilirubin moves from the bile canaliculi of the liver to the gall bladder where it is excreted into the small intestine during digestion. High levels of bilirubin can result in jaundice and may indicate liver disease, blood disorders, or blockage of the bile ducts.

Application

Bilirubin Assay Kit has been used to measure the total bilirubin concentration in samples.

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of of both total and conjugated bilirubin in serum and plasma samples.

Principle

This assay, based on the Jendrassik-Grof method, utilizes the reaction of bilirubin with diazotized sulfanilic acid resulting in a colorimetric product measured at 530 nm, proportionate to the bilirubin present in the sample. This assay kit measures both total and conjugated bilirubin.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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