MAK154

Fluorometric Sphingomyelin Assay Kit

sufficient for 100 fluorometric tests

• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• usage: sufficient for 100 fluorometric tests
• detection method: fluorometric
• relevant disease(s): cancer
• storage temp.: −20°C

Description

General description

Sphinogmyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomylein is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity.

Suitability

This kit is suitable for the detection of sphingomyelin in a variety of samples such as blood and cell extracts.

Principle

Sphingomyelin is determined by a coupled enzyme assay, in which the hydrolysis of sphingomyelin by sphingomyelinase produces phosphocholine, which results in a fluorescent product (λ_ex =540/λ_em = 590 nm) proportional to the Sphingomyelin present.

Safety Information

• Pictograms: GHS08,GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H317,H318,H334,H410
• Precautionary Statements: P261 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids