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N7763

Sigma-Aldrich

4-Nitrophenyl α-L-rhamnopyranoside

≥98% (TLC), powder

Synonym(s):

p-Nitrophenyl 6-deoxy-α-L-mannopyranoside

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About This Item

Empirical Formula (Hill Notation):
C12H15NO7
CAS Number:
Molecular Weight:
285.25
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

product name

4-Nitrophenyl α-L-rhamnopyranoside, powder

Assay

≥98% (TLC)

Quality Level

form

powder

solubility

ethanol: soluble 49.00-51.00 mg/mL

storage temp.

−20°C

SMILES string

CC1OC(Oc2ccc(cc2)N(=O)=O)C(O)C(O)C1O

InChI

1S/C12H15NO7/c1-6-9(14)10(15)11(16)12(19-6)20-8-4-2-7(3-5-8)13(17)18/h2-6,9-12,14-16H,1H3

InChI key

YILIDCGSXCGACV-UHFFFAOYSA-N

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Application

4-Nitrophenyl α-L-rhamnopyranoside has been used as a substrate to determine β-glucosidase activity. It has also been used as a substrate to measure α-L-rhamnosidase activity of Oenococcus oeni and Aspergillus terreus.

Biochem/physiol Actions

4-Nitrophenyl α-L-rhamnopyranoside is a substrate for determining α-L-rhamnosidase activity. The hydrolysis of 4-Nitrophenyl α-L-rhamnopyranoside by the enzyme yields 4-nitrophenol, measured at 405 nm spectrophotometrically.

Substrates

Chromogenic substrate for naringinase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The yeast Wickerhamomyces anomalus AS1 secretes a multifunctional exo-beta-1, 3-glucanase with implications for winemaking
Schwentke J, et al.
Yeast, 31(9), 349-359 (2014)
Sandra Garrigues et al.
iScience, 25(4), 104065-104065 (2022-04-02)
Efficient utilization of agro-industrial waste, such as sugar beet pulp, is crucial for the bio-based economy. The fungus Aspergillus niger possesses a wide array of enzymes that degrade complex plant biomass substrates, and several regulators have been reported to play
Carmen Maturano et al.
World journal of microbiology & biotechnology, 33(8), 151-151 (2017-07-05)
Autochthonous Oenococcus oeni strains (MS9, MS20 and MS46) with good malolactic performance and yielding adequate diacetyl levels, were selected to investigate the effect of synthetic and grape glycosides on bacterial growth, substrate utilization and β-glucosidase (βGlu), α-arabinofuranosidase (αAra) and α-rhamnopyranosidase
Purification and characterization of an alpha-L-rhamnosidase from Pichia angusta X349
Yanai T and Sato M
Bioscience, Biotechnology, and Biochemistry, 64(10), 2179-2185 (2000)
Recombinant alpha-L-rhamnosidase from Aspergillus terreus in selective trimming of rutin
Gerstorferova D, et al.
Process Biochemistry (Oxford, United Kingdom), 47(5), 828-835 (2012)

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