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P3288

Sigma-Aldrich

Phosphate buffer pH 7.2 at 25 °C

for hematology and histology staining techniques

Synonym(s):

phosphate buffer

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About This Item

UNSPSC Code:
12352107
NACRES:
NA.47

Quality Level

form

powder

color

white

pH

7.10-7.30

application(s)

hematology
histology

storage temp.

room temp

General description

Phosphate buffer is the most commonly used component of irrigation solution during surgeries, to preserve the living tissues. It is also used in immunochemical staining procedures. Phosphate buffer serves as an important fixative for light and electron microscopy. Its buffering capacity is pH sensitive and is preferred to be used at physiological pH (7.2-7.4).

Application

Intended for use as a buffer in various Romanowsky type staining procedures, including Wright Stain, Wright Giemsa, Giemsa, May Grunwald, Jenner and Leishman. Phosphate buffer pH 7.2 at 25 °C has been used in oocyte collection from porcine ovaries.

Reconstitution

Each vial will prepare 3.8 L of buffer, 8.3 mM.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Low concentrations of MEM vitamins during in vitro maturation of porcine oocytes improves subsequent parthenogenetic development
Naruse K, et al.
Theriogenology, 67(2), 407-412 (2007)
Biological Electron Microscopy: Theory, Techniques, and Troubleshooting (2011)
Thaise L Teixeira et al.
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P21 is a protein secreted by Trypanosoma cruzi (T. cruzi). Previous studies have shown a spectrum of biological activities performed by P21 such as induction of phagocytosis, leukocyte chemotaxis and inhibition of angiogenesis. However, the activity of P21 in T.

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