Skip to Content
Merck
All Photos(1)

Documents

P3563

Sigma-Aldrich

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween® 20

Synonym(s):

1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41105319
eCl@ss:
32129211
NACRES:
NA.25

product name

Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4,

form

powder

Quality Level

200

solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

cell analysis
diagnostic assay manufacturing

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Application

Phosphate buffered saline has been used in:

  • western blotting
  • enzyme-linked immunosorbent assay (ELISA)
  • telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
  • cell isolation

Packaging

Foil pouches

Reconstitution

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Kossi Komlan et al.
PLoS neglected tropical diseases, 12(3), e0006312-e0006312 (2018-03-02)
Mass drug administration (MDA) of ivermectin has become the main intervention to control onchocerciasis or "river blindness". In Togo, after many years of MDA, Onchocerca volvulus infection has declined dramatically, and elimination appears achievable, but in certain river basins the
Telomere length assessment in human archival tissues: combined telomere fluorescence in situ hybridization and immunostaining
Meeker A K, et al.
The American Journal of Pathology, 160(4), 1259-1268 (2002)
Shorter telomeres in luminal B, HER-2 and triple-negative breast cancer subtypes
Heaphy C M, et al.
Modern Pathology, 24(2), 194-194 (2011)
Expression of the galectin-9-Tim-3 pathway in glioma tissues is associated with the clinical manifestations of glioma
Liu Z, et al.
Oncology Letters, 11(3), 1829-1834 (2016)
The alternative lengthening of telomeres phenotype in breast carcinoma is associated with HER-2 overexpression
Subhawong A P, et al.
Modern Pathology, 22(11), 1423-1423 (2009)
View All Related Papers

Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service