SAB4200188

Anti-U1 snRNP C (U1C) antibody, Rat monoclonal

clone 4H12, purified from hybridoma cell culture

• Synonym(s): Anti-Snrp1c, Anti-Snrpc, Anti-U1 small nuclear ribonucleoprotein C, Anti-U1-C, Anti-U1C
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: 4H12, monoclonal
• form: buffered aqueous solution
• mol wt: ~20 kDa
• species reactivity: human, mouse, rat, hamster, monkey
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 1-2 μg/mL using HeLa or COS7 or CHO cell extracts
• isotype: IgG2a
• UniProt accession no.: P09234
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... SNRPC(6631) ; mouse ... Snrpc(20630) ; rat ... LOC685273(685273)

Description

General description

Monoclonal Anti-U1 snRNP C (U1C) (rat IgG2a isotype) is derived from the hybridoma 4H12 produced by the fusion of mouse myeloma cells (SP2) and splenocytes from a rat immunized with mouse U1C protein. Small nuclear ribonucleoprotein polypeptide C (U1C) is mapped to human chromosome 6p21.31.

The U1 snRNP complex contains the U1 snRNA molecule and the U1 snRNP specific proteins U1-70K, U1A and U1C, plus a common set of eight proteins, called Sm proteins U1A and U1-70K contain RNA binding domains and interact with naked snRNA on their own.

Specificity

Monoclonal Anti-U1 snRNP C (U1C) recognizes human, monkey, mouse, rat, and hamster U1C.

Immunogen

mouse U1C protein fusion protein

Application

Anti-U1 snRNP C (U1C) antibody, Rat monoclonal has been used for:

• immunoblotting
• immunofluorescence
• immunoprecipitation
• immunocytochemistry

Biochem/physiol Actions

The U1 small nuclear ribonucleoprotein particle (snRNP) has an important function in the early formation of the spliceosome, the multicomponent complex in which pre-mRNA splicing takes place. The binding of U1C to the U1snRNP particle is dependent on protein-protein interactions between U1C and U1-70K as well as U1C and the common Sm proteins.

U1A and U1−70K contain RNA binding domains and interact with naked snRNA on their own. In cultured HeLa cells, mutant U1C proteins that are not able to bind to the U1 snRNP do not accumulate in the nucleus, indicating that nuclear accumulation of U1C is due to incorporation of the protein into the U1 snRNP.

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

Store at -20 °C. For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable