SHC003V

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles

Green fluorescent protein marker to monitor transduction efficiency

• Synonym(s): MISSION^®, MISSION^® TurboGFP^™ Control Transduction Particles
• UNSPSC Code: 41106609
• NACRES: NA.51

Properties

• Quality Level: 100; 200
• product line: MISSION^®
• concentration: ≥1x10⁶ VP/ml (via p24 assay)
• technique(s): capture ELISA: 10⁶ TU/mL using p24
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.

TurboGFP^™ is an improved variant of the green fluorescent protein copGFP cloned from the copepoda Pontellina plumata. The TurboGFP^™ transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP^™ (SHC003). It is a positive control to monitor transduction efficiency.

Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 10⁶ TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.

When conducting experiments using MISSION^® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Application

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboBeads is a trademark of TurboBeads LLC

TurboGFP is a trademark of Evrogen Co.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves