SRP3115
MIP-1 beta (CCL4) human
recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
ACT-2, CCL4
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
human
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
mol wt
7.6 kDa
packaging
pkg of 10 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... CCL4(6351)
General description
Both MIP-1α and MIP-1β (macrophage inflammatory proteins-1α and β) are structurally and functionally related CC chemokines. They are secreted by activated human monocytes and peripheral blood lymphocytes. The gene encoding this protein is localized on human chromosome 17q12. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells. Recombinant human MIP-1β is a 7.6kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Biochem/physiol Actions
MIP-1α and MIP-1β (macrophage inflammatory proteins-1α and β) participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and natural killer (NK) cells. While both MIP-1α and MIP-1β exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1α selectively attracting CD8+ lymphocytes and MIP-1β (also known as C-C motif chemokine ligand 4) selectively attracting CD4+ lymphocytes. Additionally, MIP-1α and MIP-1β have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells.
Sequence
APMGSDPPTA CCFSYTARKL PHNFVVDYYE TSSLCSQPAV VFQTKRGKQV CADPSESWVQ EYVYDLELN
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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S D Wolpe et al.
The Journal of experimental medicine, 167(2), 570-581 (1988-02-01)
We report the identification and purification of a new inflammatory monokine synthesized by the macrophage tumor cell line RAW 264.7 in response to endotoxin. This monokine, which we term "macrophage inflammatory protein" (MIP), is a doublet with an apparent molecular
Biology of the RANTES/SIS cytokine family.
T J Schall
Cytokine, 3(3), 165-183 (1991-05-01)
CCL3L1 and CCL4L1 chemokine genes are located in a segmental duplication at chromosome 17q12.
Modi WS
Genomics, 83(4), 735-738 (2004)
Identification of human macrophage inflammatory proteins 1alpha and 1beta as a native secreted heterodimer.
Guan E
The Journal of Biological Chemistry, 276(15), 12404-12409 (2001)
MicroRNA-125b modulates inflammatory chemokine CCL4 expression in immune cells and its reduction causes CCL4 increase with age.
Cheng NL
Aging Cell, 14(2), 200-208 (2015)
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