T6397

3,3′,5-Triiodo-L-thyronine sodium salt

powder, BioReagent, suitable for cell culture

• Synonym(s): Liothyronine, O-(4-Hydroxy-3-iodophenyl)-3,5-diiodo-L-tyrosine sodium salt, T₃
• Empirical Formula (Hill Notation): C₁₅H₁₁I₃NNaO₄
• CAS Number: 55-06-1
• Molecular Weight: 672.96
• Beilstein: 8179867
• EC Number: 200-223-5
• MDL number: MFCD00002594
• UNSPSC Code: 12352209
• PubChem Substance ID: 24900372
• NACRES: NA.77

Properties

• biological source: synthetic
• Quality Level: 200
• product line: BioReagent
• Assay: ≥95%
• form: powder
• potency: 0.02-50 ng/mL (lit.)
• technique(s): cell culture | mammalian: suitable
• solubility: ethanol/1 M HCl (4:1): 10 mg/mL, clear, colorless to yellow
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: [Na+].N[C@@H](Cc1cc(I)c(Oc2ccc(O)c(I)c2)c(I)c1)C([O-])=O
• InChI: 1S/C15H12I3NO4.Na/c16-9-6-8(1-2-13(9)20)23-14-10(17)3-7(4-11(14)18)5-12(19)15(21)22;/h1-4,6,12,20H,5,19H2,(H,21,22);/q;+1/p-1/t12-;/m0./s1
• InChI key: SBXXSUDPJJJJLC-YDALLXLXSA-M
• Gene Information: human ... THRA(7067) , THRB(7068) ; rat ... Thra(81812) , Thrb(24831)

Description

General description

3,3′,5′-Triiodo-L-thyronine (T₃) is an endocrine hormone produced by the thyroid gland and is regarded as an active thyroid hormone at cellular level. Thyroxine (T₄) serves as a precursor for T₃ and is generated in extrathyroidal tissues.

Application

3,3′,5-Triiodo-L-thyronine sodium salt has been used for inducing thyroid hormone manipulations in mice. It has also been used In cell culturing of hippocampal, retinal, proximal tubule and glomerular explant.

Biochem/physiol Actions

3,3′,5′-Triiodo-L-thyronine (T₃) increases the rates of protein synthesis, stimulates the breakdown of cholesterol and effects embryonic development. In cell culture, T₃ regulates cell differentiation and protein expression. The interaction of T₃ with nonhistone proteins in the chromatin initiates the thyroid hormone activity. Thyroid hormones regulate the differentiation and development of tissues and affect a number of metabolic processes. The level of circulating T₃ hormone is reduced in hypothyroidism.

Physical form

powder-20 °C; stock-frozen in working aliquots, avoid repeated freeze/thaw, solution stable 2-8 °C, 30 days

Reconstitution

To prepare 20 μg/ml stock solution: add 1ml 1N NaOH per mg 3,3′, 5-triiodo-L-thyronine; gently swirl to dissolve. To this, add 49 ml sterile medium per ml 1N NaOH added.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves