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577528-U

Supelco

BIOshell A160 Peptide Phenyl-Hexyl (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

Synonym(s):

BIOshell A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

Product Name

BIOshell A160 Peptide Phenyl-Hexyl, 2.7 μm HPLC Column, column L × I.D. 15 cm × 2.1 mm

material

stainless steel column

Agency

suitable for USP L11

product line

BIOshell

feature

endcapped

packaging

1 ea of

extent of labeling

4.7% Carbon loading

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

column L × I.D.

15 cm × 2.1 mm

surface area

90 m2/g

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General description

BIOshell A160 Peptide Phenyl-Hexyl is a high-speed, high-performance liquid chromatography column based on a 160 Å Fused-Core® particle design. The particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency for high molecular weight solutes (up to 20 kDa) due to the shallow diffusion paths in the 0.5 μm thick porous shell and the small overall particle size of 2.7 μm. The end-capped, phenyl-hexyl bonded phase of the Peptide Phenyl-Hexyl provides a stable, reversed phase packing with a pore structure and pore size that is optimized for reversed-phase HPLC separations of peptides and polypeptides, using typical acidic mobile phases favored for proteomic applications.

Analysis Note

The target analytes include peptides and polypeptides < 20 kDa.

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

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Articles

This application note compares three different column chemistries from the BIOshell™ line of columns with superficially porous particles to evaluate their performance in peptide mapping.

Related Content

HPLC separates and identifies large biomolecules like proteins and peptides by pumping the sample through a sorbent-packed column.

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