235420

Caspase-3 Inhibitor I

The Caspase-3 Inhibitor I, also referenced under CAS 169332-60-9, controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Caspase-3 Inhibitor I, CPP32/Apopain Inhibitor, Ac-DEVD-CHO
• Empirical Formula (Hill Notation): C₂₀H₃₀N₄O₁₁
• CAS Number: 169332-60-9
• Molecular Weight: 502.47
• MDL number: MFCD00671412
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥90% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: DMSO: 5 mg/mL; water: soluble
• shipped in: ambient
• storage temp.: −20°C
• InChI: 1S/C20H30N4O11/c1-9(2)17(20(35)22-11(8-25)6-15(29)30)24-18(33)12(4-5-14(27)28)23-19(34)13(7-16(31)32)21-10(3)26/h8-9,11-13,17H,4-7H2,1-3H3,(H,21,26)(H,22,35)(H,23,34)(H,24,33)(H,27,28)(H,29,30)(H,31,32)/t11-,12-,13-,17-/m0/s1
• InChI key: UMBVAPCONCILTL-MRHIQRDNSA-N

Description

General description

A specific and reversible inhibitor of CPP32/Apopain/Yama (IC₅₀ = 0.2 nM). A member of the ICE/CED-3 family of cysteine proteases, which are important in apoptosis.

A very potent, specific, and reversible inhibitor of caspase-3 (IC₅₀ = 200 pM), caspase-6, caspase-7, caspase-8, and caspase-10.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Capase-3, capase-6, capase-7, capase-8, capase-10

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 200 pM against caspase-3

Packaging

Yes

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Glu-Val-Asp-CHO

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Garcia-Calvo, M., et al. 1998. J. Biol. Chem. 273, 32608.
Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
Nicholson, D.W. 1996. Nature Biotech. 14, 297.
Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
Nicholson, D.W., et al. 1995. Nature376, 37.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable