ABN458
Anti-Choline Transporter Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
High affinity choline transporter 1, Hemicholinium-3-sensitive choline transporter, CHT, Solute carrier family 5 member 7
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat, mouse
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SLC5A7(60482)
General description
The protein named Choline Transporter or Hemicholinium-3-sensitive choline transporter (CHT), and encoded by the gene SLC5A7/CHT1, is the high affinity choline transporter in neurons. Choline is the rate limiting step in acetylcholine synthesis an important neurotransmitter. Acetylcholine is a neurotransmitter of the central and peripheral nervous system that regulates a variety of autonomic, cognitive, and motor functions. The choline transporter is localized to the cell membrane and is expressed in cholinergic neurons particularly in the putamen, spinal cord and medulla. Defects in the choline transporter may be associated with a syndrome named distal spinal muscular atrophy with vocal cord paralysis. The choline transporter protein is a Na+ and Cl- dependent transporter that can be inhibited by nanomolar concentrations of the chemical compound hemicholinium 3, a compound often used to deplete acetylcholine stores.
Immunogen
GST-tagged recombinant protein corresponding to the C-terminus of human Choline Transporter.
Application
Anti-Choline Transporter Antibody is a highly specific rabbit polyclonal antibody, that targets Choline Transporter & has been tested in western blotting & IHC (Paraffin).
Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Choline Transporter in rat spinal cord and human cerebral cortex tissue.
Quality
Evaluated by Western Blotting in rat spinal cord tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Choline Transporter in 10 µg of rat spinal cord tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Choline Transporter in 10 µg of rat spinal cord tissue lysate.
Target description
~70-75 kDa observed. Uniprot states MW at ~63kDa but lists one glycosylation site which adds ~5kDa to the total MW.
Linkage
Replaces: AB5960;AB5966
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Eryn Donovan et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(16), 3426-3444 (2022-03-03)
Transport of choline via the neuronal high-affinity choline transporter (CHT; SLC5A7) is essential for cholinergic terminals to synthesize and release acetylcholine (ACh). In humans, we previously demonstrated an association between a common CHT coding substitution (rs1013940; Ile89Val) and reduced attentional
Ajeesh Koshy Cherian et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(11), 2947-2959 (2017-02-15)
Some rats [sign-trackers (STs)] are prone to attribute incentive salience to reward cues, which can manifest as a propensity to approach and contact pavlovian cues, and for addiction-like behavior. STs also exhibit poor attentional performance, relative to goal-trackers (GTs), which
Ajeesh Koshy Cherian et al.
Neurochemistry international, 108, 410-416 (2017-06-05)
The synaptic uptake of choline via the high-affinity, hemicholinium-3-dependent choline transporter (CHT) strongly influences the capacity of cholinergic neurons to sustain acetylcholine (ACh) synthesis and release. To advance research on the impact of CHT capacity in humans, we established the
Maximilian I Pinkham et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 312(3), R368-R379 (2017-01-06)
Heart failure is characterized by the loss of sympathetic innervation to the ventricles, contributing to impaired cardiac function and arrhythmogenesis. We hypothesized that renal denervation (RDx) would reverse this loss. Male Wistar rats underwent myocardial infarction (MI) or sham surgery
Fumiaki Yokoi et al.
IBRO neuroscience reports, 11, 1-7 (2021-07-01)
DYT-TOR1A or DYT1 early-onset generalized dystonia is an inherited movement disorder characterized by sustained muscle contractions causing twisting, repetitive movements, or abnormal postures. The majority of the DYT1 dystonia patients have a trinucleotide GAG deletion in DYT1/TOR1A. Trihexyphenidyl (THP), an
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