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HCCB4MAG-58K

Millipore

MILLIPLEX® Human Circulating Cancer Biomarker Magnetic Bead Panel 4 - Cancer Multiplex Assay

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About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

species reactivity

human

Quality Level

manufacturer/tradename

Milliplex®

assay range

accuracy: 80-110%
standard curve range: 0.1-100 ng/mL
(L1CAM)

standard curve range: 0.1-100 ng/mL
(Mesothelin)

standard curve range: 0.3-250 ng/mL
(Hepsin)

standard curve range: 1.4-1,000 ng/mL
(CD44)

standard curve range: 1.4-1,000 ng/mL
(Transglutaminase 2 (TGM2))

standard curve range: 13.7-10,000 pg/mL
(EpCAM)

standard curve range: 13.7-10,000 pg/mL
(Midkine)

standard curve range: 2.7-2,000 ng/mL
(ALDH1A1)

standard curve range: 20.6-15,000 pg/mL
(Carbonic Anhydrase IX (CA9))

standard curve range: 27.4-20,000 pg/mL
(Kallekrein-6)

inter-assay cv: <10%
intra-assay cv: <20%

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

wet ice

General description

Circulating cancer biomarkers give insight beyond intracellular oncogenesis and into how the body responds to the presence of a tumor, the dysregulation of homeostasis, and the relationship between a tumor and its environment. Multianalyte panels have become essential tools that enable researchers to measure cancer biomarkers simultaneously, teasing out these relationships and applying them to understand tumor biology.

MILLIPLEX® Human Circulating Cancer Biomarker Panel 4 is a 10-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum and plasma samples: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), and Transglutaminase 2 (TGM2). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Circulating Cancer
MILLIPLEX® MAP Human Circulating Cancer Biomarker Panel 4 is a Bead-Based Multiplex Assay using the Luminex xMAP technology, enabling the simultaneous analysis of multiple cancer biomarkers in human serum, plasma, and cellular tissue culture samples.

Application

  • Analytes: Aldheyde dehydrogenase 1 Family, Member A1 (ALDH1A1), Carbonic Anhydrase IX (CA9), CD44, Epithelial cell adhesion molecule (EpCAM), Hepsin, Kallikrein-6, Mesothelin, Midkine, Neural cell adhesion Molecule L1 (L1CAM), Transglutaminase 2 (TGM2)
  • Recommended Sample Type: Human serum, plasma or tissue/cell/tumor culture supernatant
  • Recommended Sample Dilution: 25 μL per well of 1:5 diluted serum or plasma can be used. Tissue culture supernatant may require a dilution with an appropriate control medium prior to assay
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • NOTE: min DC for CA9, Midkine, Kallikrein 6 and EpCAM are in pg/mL while the rest of the analyte min DC are in ng/mL
  • Research Category: Cancer
  • Research Subcategory: Immune Response, Alzheimer′s Disease, Inflammation, Oncology, Tumor Markers

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.
Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Imad Tarhoni et al.
Journal of immunoassay & immunochemistry, 39(1), 84-98 (2018-01-09)
Angiogenesis is associated with tumor progression in a range of malignancies. Herein, we develop custom immunobead assays for several mechanistically important targets and evaluated these against sera from cohorts of non-small cell lung cancer (NSCLC) patients. Antigen "capture" antibodies for

Related Content

Cancer biomarker detection through MILLIPLEX® multiplex cancer assays can accelerate studies of tumorigenic processes helping advance cancer research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service