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51800C

Sigma-Aldrich

Claycomb Medium

without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

HL-1 Medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

description

For research or for further manufacturing use.

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Application

Claycomb Medium, named after Dr. William Claycomb who established the HL-1 cell line, is specifically designed for the growth of murine cardiomyocytes. HL-1 is the first cell line established that can maintain the differentiated cardiomyocyte phenotype and contractile activity in vitro. The HL-1 cell line can be used for the study of cardiac cell hypertrophy that follows myocardial infarction, the testing of novel cardiac therapeutic drugs and treatments, the production of high levels of cardiac proteins and the study of mature cardiomyocyte specific genes. Claycomb Medium, when supplemented with 100 μM norepinephrin, 10% fetal bovine serum (FBS) and 4 mM L-glutamine, will maintain the HL-1 cell line and the mature cardiomycyte behavior. While observing the HL-1 cells under light microscopy, individual and groups of cells can be observed contracting, becoming more frequent as the cardiomyocytes reach confluency.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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L A Sikkink et al.
Cell death & disease, 1, e98-e98 (2010-01-01)
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Jan Hendrik van Weerd et al.
eLife, 9 (2020-07-17)
Genome-wide association studies have implicated common genomic variants in the gene desert upstream of TBX3 in cardiac conduction velocity. Whether these noncoding variants affect expression of TBX3 or neighboring genes and how they affect cardiac conduction is not understood. Here
Malou van den Boogaard et al.
Cell reports, 28(10), 2704-2714 (2019-09-05)
The human ether-a-go-go-related gene KCNH2 encodes the voltage-gated potassium channel underlying IKr, a current critical for the repolarization phase of the cardiac action potential. Mutations in KCNH2 that cause a reduction of the repolarizing current can result in cardiac arrhythmias
Yaopeng Hu et al.
Pflugers Archiv : European journal of physiology, 473(3), 507-519 (2021-01-05)
TRPM4 is a Ca2+-activated nonselective cation channel involved in cardiovascular physiology and pathophysiology. Based on cellular experiments and numerical simulations, the present study aimed to explore the potential arrhythmogenicity of CaMKII-mediated TRPM4 channel overactivation linked to Ca2+ dysregulation in the
Yunchang Liu et al.
Cell death & disease, 11(9), 756-756 (2020-09-17)
In this study, we first established the doxorubicin-induced cardiotoxicity (DIC) model with C57BL/6 mice and confirmed cardiac dysfunction with transthoracic echocardiography examination. RNA-sequencing was then performed to explore the potential mechanisms and transcriptional changes in the process. The metabolic pathway

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