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D9170

Sigma-Aldrich

Dimethyl sulfoxide

PCR Reagent

Synonym(s):

DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC Code:
12191502
PubChem Substance ID:
NACRES:
NA.25

grade

PCR Reagent

Quality Level

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

Assay

≥99.5%

form

liquid

autoignition temp.

573 °F

shelf life

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

packaging

vial of 1 mL

technique(s)

PCR: suitable

impurities

≤0.1% water
water

color

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

suitability

suitable for PCR

application(s)

agriculture

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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General description

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs). Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

Application

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture and as PCR facilitator.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, Todd C
Journal of Visualized Experiments, (63) (2012)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)

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