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Supelco

SUPELCOSIL LC-18-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

High Purity C18 HPLC Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

SUPELCOSIL LC-18-DB HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

Agency

suitable for USP L1

product line

SUPELCOSIL

feature

endcapped

manufacturer/tradename

SUPELCOSIL

packaging

1 ea of

extent of labeling

11.0% Carbon loading

parameter

0-70 °C temperature
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable

L × I.D.

25 cm × 4.6 mm

surface area

170 m2/g

surface coverage

surface coverage 3.1 μmol/m2

matrix

silica gel, spherical particle platform
fully porous particle

matrix active group

C18 (octadecyl) phase

particle size

5 μm

pore size

120 Å

operating pH

2-7.5

application(s)

food and beverages

compatibility

for use with PTC-AA

separation technique

reversed phase

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General description

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide shorter retention, better peak shape, and higher efficiency for organic bases than can be obtained on other Type A silica reversed-phase columns.

Application


  • OPTIMIZATION AND VALIDATION OF HPLC METHOD FOR TETRAMETHRIN DETERMINATION IN HUMAN SHAMPOO FORMULATION.: This study discusses the optimization and validation of a high-performance liquid chromatography (HPLC) method using SUPELCOSIL LC-18-DB HPLC Column for the analysis of tetramethrin in shampoo formulations (Zeric Stosic et al., 2016).

  • Mass spectrometric characterization and HPLC determination of the main urinary metabolites of nimesulide in man.: Details a robust approach for identifying and quantifying nimesulide metabolites via HPLC, underscoring the column′s applicability in complex biological matrices (Carini et al., 1998).


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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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E Terjéki et al.
Journal of pharmaceutical and biomedical analysis, 24(5-6), 913-920 (2001-03-15)
RGH-1756 (1-(2-methoxy-phenyl)-4-(4-[4-(6-imidazo[2,1-b]-thiazolyl)-phenoxy]-butyl)-piperazine dimethansulphonate) is a novel atypical antipsychotic candidate of Gedeon Richter Ltd. A new HPLC method has been developed and validated for the quantitative determination of RGH-1756 in dog and rat plasma. The compound and the internal standard are
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 755(1-2), 331-335 (2001-06-08)
A HPLC-UV determination of loratadine in human plasma is presented. After simple liquid-liquid extraction with 2-methylbutane-hexane (2:1) and evaporation of organic phase the compounds were re-dissolved in 0.01 M HCl, evaporated again and finally separated on a Supelcosil LC-18-DB column.
J S Patrick et al.
Analytical biochemistry, 199(1), 125-131 (1991-11-15)
A rapid, isocratic method for the determination of tryptophan in Escherichia coli fermentation broths by reversed-phase HPLC is described. Tryptophan can be measured in fermentations containing either chemically defined media or media with hydrolyzed protein supplements. The procedure was rugged
Xinghua Sun et al.
Anticancer research, 25(1A), 59-62 (2005-04-09)
A rapid, sensitive and specific high-performance liquid chromatographic (HPLC) method for the separation and quantification of L-methionine in plasma has been developed. After derivatization of plasma amino acids with o-phthalaldehyde (OPA), a 50 microl sample was loaded on a reversed-phase
S L Bramer et al.
Journal of pharmaceutical and biomedical analysis, 26(4), 637-650 (2001-08-23)
An LC/MS/MS method for the simultaneous determination of cilostazol, a quinolinone derivative, and three active metabolites, OPC-13015, OPC-13213, and OPC-13217, in human plasma was developed and validated. Cilostazol, its metabolites, and the internal standard, OPC-3930 were extracted from human plasma

Protocols

Amino Acid Analysis is the suitable tool for precise determination of protein quantities but also provides detailed information regarding the relative amino.

Chromatograms

application for SPE, application for HPLC

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