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BIOshell lgG 1000 Å C4 (2.7 μm) HPLC Columns

L × I.D. 10 cm × 2.1 mm, HPLC Column

Synonym(s):

BIOshell lgG C4 Chromatography

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About This Item

UNSPSC Code:
41115709
NACRES:
SB.52

product name

BIOshell lgG 1000 Å C4, 2.7 μm HPLC Column, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

material

stainless steel hardware

Quality Level

100

Agency

suitable for USP L26

description

Shell thickness (0.5 μm)
Solid core (1.7 μm)

product line

BIOshell

feature

endcapped

specific analyte(s)

IgG

manufacturer/tradename

BIOshell

packaging

1 ea of

extent of labeling

0.6% carbon loading

parameter

1000 bar pressure (14500 psi)
90 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

10 cm × 2.1 mm

surface area

22 m2/g

matrix

spherical silica particle platform
superficially porous particle

matrix active group

C4 (butyl) phase

particle size

2.7 μm

pore size

1000 Å

operating pH range

2-9

separation technique

reversed phase

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General description

BIOshell IgG 1000Å C4 is a high-speed, high-performance liquid chromatography column based on a new wide-pore (1000Å) Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thin porous shell and the small overall particle size of 2.7-microns. The densely bonded, extensively endcapped dimethylbutyl stationary phase of BIOshell IgG 1000Å C4 provides a stable, reversed-phase packing that can be used for separating high molecular weight compounds such as proteins.

Application

UHPLC Analysis of Trastuzumab on BIOshell IgG 1000 Å C4, 2.7 μm
UHPLC Analysis of IgG2 on BIOshell IgG 1000 Å C4, C18, and Diphenyl, 2.7 μm

Legal Information

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

antibody

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guard cartridge

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related product

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Storage Class Code

13 - Non Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
USMJS001673
USMJS001672
USMJS001671
USMJS001670
USMJS001669

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Articles

Protein Fingerprinting of a Viral Vector, AAV5

Method development for protein fingerprinting of AAV serotype 5 using both intact mass analysis and peptide mapping, to determine critical quality attributes for gene therapy, utilizing three different columns.

HPLC Columns for Antibody and Large Protein Analysis

BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa.

Protocols

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

BIOshell™ IgG 1000 Å C4 UHPLC Column for Improved Biomacromolecule Separations

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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