1.00432

ChromoCult^® Listeria Agar Selective-Supplement

for Listeria spp., pkg of 10 vials, for the preparation of ChromoCult^® Listeria agar (base)

• Synonym(s): Listeria Selective Supplement
• UNSPSC Code: 41106212
• NACRES: NA.74

Properties

• Agency: ISO 11290
• Quality Level: 100
• description: ChromoCult™ Listeria agar (base) acc. OTTAVIANI and AGOSTI (1.00427)
• form: lyophilized powder
• composition: Amphotericin B, 5 mg per vial ; Ceftazidime, 10 mg per vial ; Nalidixic acid sodium salt, 10 mg per vial ; Polymyxin B sulphate, 38350 (U per vial)
• packaging: pkg of 10 vials
• technique(s): microbiological culture: suitable
• pH: 6.2 (20 °C in H₂O, Aqueous solution)
• application(s): food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: Listeria spp.

Description

General description

ChromoCult^® Listeria Agar Selective-Supplement contains a mixture of four antibiotics in lyophilized form. It largely inhibits the growth of accompanying bacteria by selective enrichment of Listeria spp. Amphotericin B reduces the growth of yeasts and molds. Polymyxin B inhibits the growth of Gram-negative bacteria. While nalidixic acid sodium salt mainly inhibits the growth of Gram-negative bacteria, it also inhibits some Gram-positive bacteria. Ceftazidime has a broad-spectrum activity against both Gram-negative and Gram-positive bacteria.

Application

FRASER Listeria Selective Supplement is an additive for the preparation of ChromoCult^® Listeria agar (base) for the selective growth of Listeria spp.

Linkage

Replaces: 1.00432.0017

Analysis Note

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 hrs. 37°C aerobic; other strains at least 48 hrs.

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334,H351,H373
• Precautionary Statements: P202 - P260 - P272 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Carc. 2 - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3