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CDUFBI001

Milli-Q

Biopak® Polisher

Ultrafilter for the production of pyrogen-, nuclease-, and bacteria-free water

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About This Item

UNSPSC Code:
41104212
eCl@ss:
33050190
NACRES:
NA.21

material

polysulfone (Ultrafiltration Hollow Fibers)

Quality Level

300

feature

removal of pyrogen (endotoxins), nuclease and bacteria in water

packaging

pkg of 1 unit

manufacturer/tradename

BioPak®

impurities

<0.001 EU/mL Pyrogens (endotoxins)
<0.01 ng/mL RNases
<0.1 cfu/mL Bacteria
<4 pg/μL DNases

compatibility

for use with Direct-Q® UV
for use with Direct-Q®
for use with Elix® Advantage
for use with Milli-Q® Direct
for use with Milli-Q® Reference
for use with Simplicity® UV
for use with Simplicity®
for use with Synergy® UV
for use with Synergy®

shipped in

ambient

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General description

The Biopak® Polisher is an ultrafiltration cartridge designed to produce water suitable for molecular biology, biochemistry and cell culture when fed with Milli-Q® purified water. It is validated for continuous use for 90 days.

Application

Biopak® Polisher is recommended for a wide range of laboratory applications such as molecular biology, cell culture and biochemistry, where bacteria, pyrogen and/or nuclease levels must be controlled. This includes bloodstream bacterial infections (BSIs) research, electrochemical biosensor development, endotoxin adsorption studies, antimicrobial testing and mRNA and protein detection.

Features and Benefits

  • Minimizes risk of nuclease, pyrogen and bacteria contamination.
  • Eliminates the need for toxic agents such as DEPC, therefore improving safety, saving time and reducing costs.
  • Care-free maintenance: The polisher is easily installed and replaced; does not require sanitization.

Components

Ultrafiltation hollow fibers

Other Notes

Directions for Use

  • Organism Retention: Bacteria and pyrogens
  • Mode of Action: Ultrafiltration
  • Application: Cell culture media, molecular biology
  • Intended Use: Bioburden reduction
  • Instructions for Use: This item provides water filtered through ultrafiltration hollow fibers. Refer to insert, section "replacement" in the product package.
  • Storage Statement: Store in a dry location.
  • Disposal Statement: Dispose of in accordance with applicable federal, state and local regulations.

Legal Information

BIOPAK is a registered trademark of Merck KGaA, Darmstadt, Germany
DIRECT-Q is a registered trademark of Merck KGaA, Darmstadt, Germany
ELIX is a registered trademark of Merck KGaA, Darmstadt, Germany
Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany
SIMPLICITY is a registered trademark of Merck KGaA, Darmstadt, Germany
SYNERGY is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry
Arrigucci R, et al.
Nature Protocols, 12(6), 1245-1260 (2017)
A Flowthrough Assay for Rapid Bedside Stratification of
Bloodstream Bacterial Infection in Critically Ill Patients: a Pilot
Study
Jagtap P, et al.
Journal of Clinical Microbiology, 56(9), e00408-e00418 (2018)
Explicit measurement of the endotoxin adsorption efficiency detects non-Langmuir behavior at low concentrations
Kholina EG, et al.
Analytical biochemistry, 587, 113445-113445 (2019)
The importance of the assembling of DNA strands on the performance of electrochemical genosensors
Batistuti MR, et al.
Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science, 159, 105358-105358 (2020)
Tandem malonate-based glucosides (TMGs) for membrane protein structural studies
Hussain H, et al.
Scientific reports, 7(1), 1-11 (2017)

Articles

Water for Nuclease-sensitive Molecular Biology Studies

Nuclease-free water (or PCR water) is needed in molecular biology to avoid sample degradation during PCR, sequencing, etc. Ultrafiltration provides a quicker, safer, and more sustainable alternative to DEPC treatment when preparing RNase-free and DNase-free water.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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