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Key Documents

MAB2503

Sigma-Aldrich

Anti-Elastin Antibody, clone 10B8

clone 10B8, Chemicon®, from mouse

Synonym(s):

Anti-ADCL1, Anti-SVAS, Anti-WBS, Anti-WS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10B8, monoclonal

species reactivity

mouse, bovine, human, chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ELN(2006)

Specificity

Recognizes human Elastin. The antibody is also reactive with elastin from chicken, and bovine and mouse. Antibody specificity has been determined by immunoblotting of purified chick tropoelastin and immunoprecipitation of tritium-labeled chick tropoelastin from extracts of chick aorta organ cultures.

Immunogen

Tropoelastin from 17-day old lathyric chick embryo aortae, extracted an purified according to the method of Rich and Foster (1982) Meth. Enzymol. 82: 665-673.

Application

Anti-Elastin Antibody, clone 10B8 detects level of Elastin & has been published & validated for use in ELISA, IF, IP & WB.
Immunoblotting

Immunofluorescence

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Target description

66 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nandan L Nerurkar et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(9), 2277-2282 (2017-02-15)
Looping of the initially straight embryonic gut tube is an essential aspect of intestinal morphogenesis, permitting proper placement of the lengthy small intestine within the confines of the body cavity. The formation of intestinal loops is highly stereotyped within a
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Merve Celik et al.
American journal of physiology. Heart and circulatory physiology, 318(5), H1208-H1218 (2020-04-04)
In the embryonic heart, blood flow is distributed through a bilaterally paired artery system composed of the aortic arches (AAs). The purpose of this study is to establish an understanding of the governing mechanism of microstructural maturation of the AA
Ahlem Assali et al.
Development (Cambridge, England), 151(5) (2024-02-12)
EphB1 is required for proper guidance of cortical axon projections during brain development, but how EphB1 regulates this process remains unclear. We show here that EphB1 conditional knockout (cKO) in GABAergic cells (Vgat-Cre), but not in cortical excitatory neurons (Emx1-Cre)

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