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Key Documents

MAB4307

Sigma-Aldrich

Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2

clone 2H2.2, Chemicon®, from mouse

Synonym(s):

mLIF

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2H2.2, monoclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

input

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

isotype

IgG1

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L0669MAB4306L1044
biological source

mouse

biological source

mouse

biological source

mouse

biological source

goat

clone

2H2.2, monoclonal

clone

9824, monoclonal

clone

4F7.2, monoclonal

clone

polyclonal

species reactivity

mouse

species reactivity

human

species reactivity

mouse, human

species reactivity

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

isotype

IgG1

isotype

-

isotype

IgG1

isotype

-

General description

Leukemia inhibitory factor, or LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. Human LIF cDNA encodes a 202 amino acid precursor which is processed to yield a 180 amino acid, biologically active glycoprotein with an approximate molecular weight of 38-67 kDa. In addition to its involvement in the stimulation of acute-phase protein during inflammatory processes, LIF has also been implicated in promoting the survival and growth of axons in vitro. LIF signals through the LIF receptor, a heterodimer comprised of a 190 kDa LIFR subunit and the gp130 subunit common to the IL-6, IL-11, CNTF and OSM receptors. Janus tyrosine kinases, JAK1 and JAK2, are activated in response to LIF stimulation, which in turn phosphorylate Stat1 and Stat3 causing their nuclear translocation and the transcription of Stat-responsive genes.

Specificity

This antibody is reactive to E.coli and STO derived recombinant murine Leukemia Inhibitory Factor (LIF).

Immunogen

Recombinant Murine LIF.

Application

Anti-Mouse Leukemia Inhibitory Factor Antibody, clone 2H2.2 is an antibody against Mouse Leukemia Inhibitory Factor for use in ELISA & WB.
Immunoblotting: a starting dilution of 1-10 μg/mL is suggested.

EIA

Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Physical form

Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25M NaCl ph 7.6 with 0.1% Sodium Azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Analysis Note

Control
Mouse DA-1a cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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