MABC1031

Anti-TNFSF13 (APRIL) Antibody, clone A3D8

clone A3D8, from hamster(Armenian)

• Synonym(s): A proliferation-inducing ligand, APRIL, CD256, TALL-2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.43

Properties

• biological source: hamster (Armenian)
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: A3D8, monoclonal
• species reactivity: human, mouse
• technique(s): flow cytometry: suitable
• isotype: IgG
• NCBI accession no.: NP_076006
• UniProt accession no.: Q9D777
• shipped in: ambient
• target post-translational modification: unmodified
• Gene Information: mouse ... Tnfsf13(69583)

Description

General description

Tumor necrosis factor ligand superfamily member 13 (UniProt: Q9D777; also known as A proliferation-inducing ligand; APRIL; CD256) is encoded by the Tnfsf13 (also known as APRIL) gene (Gene ID: 69583) in mouse. APRIL is a secreted cytokine of the tumor necrosis family and is present in T cells, B cells, dendritic cells, monocytes, and macrophages. April exists as a functional homotrimer, but can form functional heterotrimers with B cell activating factor (BAFF). APRIL stimulates T and B cell proliferation and it exerts T and B cell regulatory effects by binding to TNFRSF17 (BCMA) and TNFRSF13B (TACI) receptors. It is also involved in monocyte/macrophage-mediated immunological processes. It also plays a role in the regulation of tumor cell growth and can induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. APRIL can serve as as a potential biomarker and its serum levels are shown to be elevated for certain cancers and expression levels elevated in cancer tissues.

Specificity

Clone A3D8 detects TNFSF13/APRIL in human peripheral blood mononuclear cells (PBMC).

Immunogen

A full length recombinant fusion protein of murine maltose binding protein (MBP) and soluble APRIL.

Application

Detect TNFSF13 using this armenian hamster monoclonal Anti-TNFSF13 (APRIL) antibody, clone A3D8, Cat. No. MABC1031, validated for use in Flow Cytometry.

Flow Cytometry Analysis: 1 µg of this antibody from a representative lot detected TNFSF13 in one million RAW264.7 cells.

Research Category
Apoptosis & Cancer

Quality

Evaluated by Flow Cytometry in human PBMC cells.

Flow Cytometry Analysis: 1 µg of this antibody detected TNFSF13 in human PBMC cells.

Target description

26.89 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified armenian hamster monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2