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39465

Millipore

Gelatin from porcine skin

suitable for microbiology, ultrahigh gel strength

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
41106212
NACRES:
NA.85

biological source

Porcine

Quality Level

form

powder

quality

ultrahigh gel strength

shelf life

limited shelf life, expiry date on the label

loss

9.5-12.5% loss on drying

transmittance

450 nm, ≥85%
620 nm, ≥95%

pH

5.20-5.60

conductivity

120-190 μS/cm at 30 °C (1%)

viscosity

5.10-5.80 mPa.s

gel strength

280-302 g BloomAOAC

solubility

H2O: 67 mg/mL at 60 °C

cation traces

Ca: ≤100 mg/kg

application(s)

microbiology

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Application

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Components

Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Caution

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentrat ion. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. Manufactured by Gelita AG

Storage Class Code

11 - Combustible Solids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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M V Nasekin et al.
Anesteziologiia i reanimatologiia, (1)(1), 59-60 (2013-07-03)
The article deals with skills improving problem of epidural anaesthesia with ultrasound control. Methods of gelatin spinal column model making, use and its economical side are discussed in the article.
Na Rae Han et al.
Biochemical and biophysical research communications, 436(3), 413-417 (2013-06-12)
Poor understanding of the differentiation of mesenchymal stem cells (MSCs) has resulted in a low differentiation yield, and has hindered their application in medicine. As a solution, priming MSCs sensitive to signaling, thus stimulating differentiation into a specific cell lineage
Takeo Furuya et al.
The journal of spinal cord medicine, 36(2), 134-139 (2013-07-03)
Besides stimulating angiogenesis or cell survival, basic fibroblast growth factor (bFGF) has the potential for protecting neurons in the injured spinal cord. To investigate the effects of a sustained-release system of bFGF from gelatin hydrogel (GH) in a rat spinal
Monica A Serban et al.
Methods in molecular biology (Clifton, N.J.), 1001, 133-143 (2013-03-16)
Tissue engineering involves the concerted action of biomaterials, cells, and growth factors. Kidney -regeneration relies on the same combination of ingredients. Here, we describe an example of gelatin-based biomaterial preparation and its evaluation in the context of kidney biocompatibility and
Panuphong Pootawang et al.
Journal of nanoscience and nanotechnology, 13(1), 589-592 (2013-05-08)
The environmental concern pays much attention to the recent cause of the global warming effect. The reduction of the chemical uses is one of many ways to avoid this crucial problem. Herein, the green process for silver nanometallic particle formation

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