41960

Vegitone infusion broth

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Brain Heart Broth, Vegitone
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: dextrose, 2.0 g/L ; disodium phosphate, 2.5 g/L ; peptone (vegetable), 10.0 g/L ; sodium chloride, 5.0 g/L ; vegetable infusion powder, 5.0 g/L ; vegetable special infusion powder, 12.5 g/L
• manufacturer/tradename: NutriSelect^® Plus
• packaging: pkg of -500 g
• storage condition: (Dry place, tightly sealed containers)
• final pH: 7.4±0.2 (25 °C)
• application(s): agriculture; clinical testing; environmental; food and beverages; life science and biopharma; sterility testing; microbiology
• storage temp.: 2-25°C
• suitability: Haemophilus spp.; nonselective for Bacteroides spp.; nonselective for Candida spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Pseudomonas spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Vegitone Infusion Broth is like Brain Heart Infusion Broth with the difference that animal peptones are replaced by plant peptones. It is a highly nutritious and well-buffered medium that supports the growth of a wide variety of microorganisms. Peptone (vegetable), Vegetable Special Infusion powder, and Vegetable Infusion powder are non-animal nutritive sources of peptides and amino acids providing nitrogen for the growth of microorganisms. Dextrose is the fermentable carbohydrate source, and sodium chloride is for the osmotic balance in the media. Disodium phosphate is used as the buffering agent.The addition of desired components allows the modification of this medium as per cultivation requirements. Sodium chloride (6.5 %) makes the medium selective for salt-tolerant bacteria like Enterococci. Adding agar (0.1 %) to the broth improves the growth of micro-aerophilic and anaerobic microorganisms. Enrichment with 10 % sheep blood allows isolation and cultivation of a wide variety of fungal species, including systemic fungi from clinical specimens. Additional gentamicin and/or chloramphenicol accommodate for selective isolation of these fungi.

Application

Vegitone Infusion Broth is employed for the propagation of fastidious pathogenic cocci and other organisms associated with blood culture work and allied pathological investigations. This medium is also suitable for inoculum preparation used in antimicrobial susceptibility tests. It is also recommended by ISO Committee under the specifications ISO 6888:1983 for the enrichment of Staphylococcus aureus.

Preparation Note

Suspend 37 g in 1000 ml distilled water. Dispense into bottles or tubes and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. For best results, the medium should be used on the day it is prepared, otherwise, it should be boiled or steamed for a few minutes and then cooled before use.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves