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Millipore

Malachite Green Broth

suitable for microbiology, NutriSelect® Basic

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About This Item

UNSPSC Code:
41171606
PubChem Substance ID:
24882432
NACRES:
NA.74

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dipotassium hydrogen phosphate, 1.1 g/L
malachite green oxalate, 0.03 g/L
meat extract, 9 g/L
meat peptone, 15 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
sample preparation
water monitoring
microbiology

suitability

selective for Pseudomonas spp.

General description

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.

Application

For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.

Preparation Note

Dissolve 25 g in 1 litre distilled water. Pour into small containers and autoclave at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4501
BCCB3572
BCBZ7005
BCBV9513
BCBS6829V

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[Is the malachite green broth according to Habs and Kirchner suitable for serving as an isolation medium for Pseudomonas aeruginosa from the water (author's transl)?].
R Schubert et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe B: Hygiene, praventive Medizin, 158(6), 583-587 (1974-04-01)
Comparative hygienic surveillance of contamination with pseudomonads in a cystic fibrosis ward over a 4-year period.
Bosshammer J et al.
Journal of Hospital Infection, 31, 261-261 (1995)
Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates.
Schwartz T et al.
FEMS Microbiology Ecology, 57, 158-158 (2006)
Characterization of mucoid Pseudomonas aeruginosa strains isolated from technical water systems.
Grobe, S., J. Wingender, and H. G. Truiper
The Journal of Applied Bacteriology, 79 (1), 94-102 (1995)

Articles

Pseudomonas Media and Tests

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

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