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Key Documents

90903

Sigma-Aldrich

Schaeffer and Fulton Spore Stain Solution A

for microscopy

Synonym(s):

Malachite Green Solution according to Schaeffer and Fulton

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About This Item

CAS Number:
2437-29-8
MDL number:
MFCD06412418
UNSPSC Code:
12171500
NACRES:
MA.02

grade

for microscopy

product line

BioChemika

form

solution

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

spore forming microorganisms

InChI

1S/2C23H25N2.3C2H2O4/c2*1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3*3-1(4)2(5)6/h2*5-17H,1-4H3;3*(H,3,4)(H,5,6)/q2*+1;;;/p-2

InChI key

CNYGFPPAGUCRIC-UHFFFAOYSA-L

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin (Schaeffer & Fulton`s Spore Stain B, Cat. No. 39955). The components and the method are also available as kit (Cat. No. 04551).

Components

Composition:
Schaeffer & Fulton`s Spore Stain A: Malachite green 50 g/l in water

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Statements

H318,H361fd,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK0823
BCCK9351
BCCJ6790
BCCH6741
BCCG1482

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Ola M Gomaa et al.
Cell biochemistry and biophysics, 65(3), 413-423 (2012-10-19)
Filamentous fungi show great promise in remediation of environmental contaminants such as industrial dyes. In the current study, Aspergillus niger (Genbank ID: JF437542) decolorized 82 % of the test dye malachite green (MG; 50 mg/l) during cultivation for 24 h.
R Sahraei et al.
Environmental monitoring and assessment, 185(7), 5817-5822 (2012-12-04)
A new spectrophotometric method is reported for the determination of nanomolar level of malachite green in surface water samples. The method is based on the catalytic effect of silver nanoparticles on the oxidation of malachite green by hexacyanoferrate (III) in
P Manoj Kumar Reddy et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 67(5), 1097-1104 (2013-02-19)
Oxidative decomposition of aqueous organic pollutant malachite green (MG) was studied in a dielectric barrier discharge reactor operated under ambient conditions. Total organic carbon content analysis confirmed the mineralization of the pollutant leading to the formation of carbon dioxide, which
Jia Zeng et al.
Biophysical journal, 104(1), 139-145 (2013-01-22)
It is shown that the nonlinear optical phenomenon known as second-harmonic generation can be used for label-free, time-resolved study of the transport of molecules through living cell membranes. The adsorption and transport of a 300-Da molecular-mass hydrophobic ion at the
Xiaofang Jia et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 18(42), 13494-13500 (2012-09-05)
This work describes a novel strategy for the highly sensitive and selective detection of cysteine (Cys) and glutathione (GSH) based on the Hg(2+)-AGRO100-malachite green (MG) complex system. The dye MG, which has a very low quantum yield in aqueous solution
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