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G7663

Sigma-Aldrich

Gelatin blocking buffer

for Western blotting, powder blend

Synonym(s):

blocking buffer

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About This Item

UNSPSC Code:
41105300
NACRES:
NA.25

grade

for molecular biology

form

powder blend

storage temp.

room temp

General description

Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).

Application

Suitable for use as a non-specific blocking agent for Western blots.

Reconstitution

Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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K A Brogden et al.
Infection and immunity, 67(8), 4256-4259 (1999-07-23)
Affinity-purified rabbit polyclonal (PAB96-1) and mouse monoclonal (1G9-1C2) antibodies to synthetic H-DDDDDDD-OH, an antimicrobial anionic peptide (AP) originally isolated from ovine pulmonary surfactant, were prepared and used to assess the concentrations of AP-like molecules in human respiratory tract samples. In
A sensitive cell-based assay for the detection of residual infectious West Nile virus.
Koldijk, M.H., et al.
Vaccine, 25, 39-39 (2007)
A Fortin et al.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 72(5-6), 239-243 (1994-05-01)
The major advantages of the horseradish peroxidase chemiluminescence (HRP-CL) immunodetection method in Western blot analysis are its high sensitivity, nonradioactive detection, economy of the primary antibody, and speed of detecting the signal. However, we observed a strong and reproducible signal
Raul Bettencourt et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 152(2), 278-289 (2008-12-02)
The interaction between microorganisms and host defense mechanisms is a decisive factor for the survival of marine bivalves. They rely on cell-mediated and humoral reactions to overcome the pathogens that naturally occur in the marine environment. In order to understand
Daisuke Tomida et al.
Investigative ophthalmology & visual science, 52(6), 3193-3199 (2011-02-08)
To study the effect of heparin on the development of laser-induced choroidal neovascularization (CNV) and to assess the underlying molecular mechanisms. Bone marrow transplantation (BMT) was conducted by intravenous injection of green fluorescence protein (GFP)-labeled bone marrow cells (1 ×
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Protocols

Gelatin Blocking Buffer for Direct Detection Southern or Western Blotting

In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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