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HPA020099

Sigma-Aldrich

Anti-PLCG2 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1

Synonym(s):

Anti-1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2, Anti-PLC-IV, Anti-PLC-gamma-2, Anti-Phosphoinositide phospholipase C, Anti-Phospholipase C-gamma-2

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

Pricing and availability is not currently available.

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

TKENNMKYWEKNQSIAIELSDLVVYCKPTSKTKDNLENPDFREIRSFVETKADSIIRQKPVDLLKYNQKGLTRVYPKGQRVDSSNYDPF

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This Item
MAB8052ZMS1110MABN12
species reactivity

human

species reactivity

human

species reactivity

adenovirus-infected cells

species reactivity

human, mouse

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

2/6, monoclonal, 20/11, monoclonal

clone

20/11, monoclonal

clone

20/11, recombinant monoclonal

clone

G2-11, monoclonal

technique(s)

ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunofluorescence: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

General description

Phospholipase C-γ-2 (PLCG2) is a multidomain protein which is expressed in mast cells and B cells. The gene encoding this protein is localized on human chromosome 16.

Immunogen

1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-2 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

Phospholipase C-γ-2 (PLCG2) hydrolyzes phosphatidylinositol-4, 5-bisphosphate to inositol-1, 4, 5 trisphosphate (IP3) and diacylglycerol. IP3 is involved in the increase of intracellular Ca2+ levels. Thus, PLCG2 is indirectly involved in increasing Ca2+ levels and mediating those pathways which require Ca2+. It is also a regulator of many inflammatory pathways. Mutations in the gene expressing PLCG2 have been associated with PLC-γ-2-associated antibody deficiency and immune dysregulation (PLAID). Steroid-sensitive nephrotic syndrome (SSNS) has also been linked to mutations in PLCG2.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST74725

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


  • Certificates of Analysis (COA)

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    Rasheed A Gbadegesin et al.
    Journal of the American Society of Nephrology : JASN, 26(7), 1701-1710 (2014-10-29)
    Steroid-sensitive nephrotic syndrome (SSNS) accounts for >80% of cases of nephrotic syndrome in childhood. However, the etiology and pathogenesis of SSNS remain obscure. Hypothesizing that coding variation may underlie SSNS risk, we conducted an exome array association study of SSNS.
    Jing Wang et al.
    Science signaling, 7(343), ra89-ra89 (2014-09-18)
    The binding of antigen to the B cell receptor (BCR) stimulates the assembly of a signaling complex (signalosome) composed initially of the kinases Lyn, spleen tyrosine kinase (Syk), and Bruton's tyrosine kinase (Btk), as well as the adaptor protein B
    Michael J Ombrello et al.
    The New England journal of medicine, 366(4), 330-338 (2012-01-13)
    Mendelian analysis of disorders of immune regulation can provide insight into molecular pathways associated with host defense and immune tolerance. We identified three families with a dominantly inherited complex of cold-induced urticaria, antibody deficiency, and susceptibility to infection and autoimmunity.
    Qing Zhou et al.
    American journal of human genetics, 91(4), 713-720 (2012-09-25)
    Whole-exome sequencing was performed in a family affected by dominantly inherited inflammatory disease characterized by recurrent blistering skin lesions, bronchiolitis, arthralgia, ocular inflammation, enterocolitis, absence of autoantibodies, and mild immunodeficiency. Exome data from three samples, including the affected father and

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