I1771

Interleukin-13 human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): IL-13
• CAS Number: 148157-34-0
• MDL number: MFCD00212209
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in E. coli
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• potency: <10.000 ng/mL ED₅₀
• mol wt: 12 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• color: white
• UniProt accession no.: P35225
• storage temp.: −20°C
• Gene Information: human ... IL13(3596)

Description

Biochem/physiol Actions

IL-13 is known to be overexpressed in glioblastoma tumors and cell lines. Elevated IL-13 expression is also observed in parasitic infections. It stimulates fibrosis in many infectious diseases, and acts as a profibrogenic factor.

Interleukin-13 is a pleiotropic cytokine produced by activated Th2 cells in the mouse and human. IL-13 is secreted mainly as an unglycosylated protein of 132 amino acids with a molecular weight of 10,000 daltons. A 4.3kb DNA fragment of the mouse IL-13 gene was sequenced and occurs as a single copy mapping a chromosome 11. In the human, a 4.6 kb DNA segment of the IL-13 gene occurs as a single copy and maps to chromosome 5. IL-13 induces B cell proliferation and also induces IgE switching. The proliferative activity of human and mouse IL-13 products are tested in culture using the human erythroleukemic cell line TF-1.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 250 μg BSA.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves