SAB5900201

Alpaca Anti-GFP, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

• Synonym(s): GFP, Green fluorescent protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: alpaca
• Quality Level: 100
• recombinant: expressed in E. coli
• conjugate: unconjugated
• antibody form: affinity purified VHH antibody
• clone: GE10, monoclonal
• Assay: >95% (SDS-PAGE)
• form: lyophilized solid
• mol wt: 13.7 kDa; ~15-18 kDa (SDS PAGE under reducing condition)
• purified by: immobilized metal affinity chromatography (IMAC)
• species reactivity: SARS coronavirus
• concentration: 1 mg/mL
• technique(s): ELISA: 1:2000-1:10000; immunofluorescence: 1:100-1:600; immunoprecipitation (IP): 0.5-4.0 μg; western blot: 1:1000-1:5000
• color: colorless
• pH: ( in PBS, pH 7.4)
• isotype: VHH
• Protein ID accession no.: BAG71039.1
• UniProt accession no.: A0A059PIQ0
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light (emission peak at 509nm) when excited by blue light (excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr (previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488 nm. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility. Alpaca derived anti-GFP VHH single-domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components.

Specificity

Green fluorescence protein (GFP) and other derivatives of GFP

Immunogen

Recombinant full length GFP protein

Application

ELISA, WB, IP, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.4

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable