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SRP3156

Sigma-Aldrich

Sonic Hedgehog human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

HHG-1

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1 ML
US$181.00
0.1 ML
US$202.00
0.5 ML
US$496.00
7 ML
US$598.00
1 ML
US$734.00

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Estimated to ship on12 February 2025


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1 ML
US$181.00
0.1 ML
US$202.00
0.5 ML
US$496.00
7 ML
US$598.00
1 ML
US$734.00

About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

US$181.00


Availability

Estimated to ship on12 February 2025


Request a Bulk Order

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

0.8-1.0 μg/mL ED50

mol wt

20.0 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

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This Item
271R-2457M-9247M-9
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

antibody form

culture supernatant

antibody form

culture supernatant

antibody form

culture supernatant

antibody form

culture supernatant

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

The sonic hedgehog protein is encoded by the SHH gene that is mapped to human chromosome 7q36. It is homologous to the Drosophila segment polarity gene hedgehog. The encoded protein is a 45kDa precursor protein that is auto-cleaved to form SSH-N (an amino-terminal 20kDa product) and SSH-C (carboxy-terminal product). SSH-N is found to be cell-associated, while SSH-C diffuses freely. [1] Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.

Application

Sonic Hedgehog human has been used:
  • To study its role in liver cancer stem cell activity.[2]
  • For neuronal differentiation.[3]
  • For cholinergic-like neurons (ChLNs) differentiation.[4]

The sonic hedgehog protein is used in neuronal induction medium as a supplement.[5]

Biochem/physiol Actions

The SHH (sonic hedgehog) gene encodes a soluble signaling protein that functions in the patterning of cell differentiation in the neural tube and limb bud. It has also been implicated in several processes, such as developing nervous system, including midbrain and ventral forebrain neuronal differentiation and neuronal precursor proliferation. In adults, it regulates progenitor function and proliferation.[6] Mutations in this gene have been associated with holoprosencephaly, a developmental defect of the forebrain and midface.[7]

Sequence

IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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    The SHH/Gli axis regulates CD 90-mediated liver cancer stem cell function by activating the IL 6/JAK 2 pathway
    Zhang K, et al.
    Journal of Cellular and Molecular Medicine, 22(7), 3679-3690 (2018)
    Direct transdifferentiation of human Wharton?s Jelly mesenchymal stromal cells into Cholinergic-like neurons
    Mendivil-Perez M, et al.
    Journal of Neuroscience Methods (2018)
    Mutations in the human Sonic Hedgehog gene cause holoprosencephaly.
    Roessler E
    Nature Genetics, 14, 357-360 (1996)
    Use of human induced pluripotent stem cell-derived neurons as a model for cerebral toxoplasmosis
    Tanaka N, et al.
    Microbes and Infection, 18(7-8), 496-504 (2016)
    Induction of midbrain dopaminergic neurons by Sonic hedgehog.
    Hynes M
    Neuron, 15, 35-44 (1995)

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